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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Actin, aortic smooth muscle, Acta2含量
- 适应物种:
小鼠
- 标记物:
Actin, aortic smooth muscle, Acta2
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Actin, aortic smooth muscle, Acta2 ELISA试剂盒
Product Name: Mouse Actin, aortic smooth muscle, Acta2 ELISA试剂盒
Product Name: Mouse Actin, aortic smooth muscle, Acta2 ELISA试剂盒
Gene Name: Mouse Actin, aortic smooth muscle, Acta2
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse Actin, aortic smooth muscle, Acta2
Alternative Name: Mouse Actin, aortic smooth muscle, Acta2
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse Actin, aortic smooth muscle, Acta2 ELISA试剂盒 allows for the in vitro quantitative determination of Mouse Actin, aortic smooth muscle, Acta2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse Actin, aortic smooth muscle, Acta2 ELISA试剂盒 has been pre-coated with an Mouse Actin, aortic smooth muscle, Acta2 antibody specific to Mouse Actin, aortic smooth muscle, Acta2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Actin, aortic smooth muscle, Acta2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Actin, aortic smooth muscle, Acta2 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Actin, aortic smooth muscle, Acta2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Models and Analysis of Atherosclerosis, Restenosis, and Aneurysm Formation in the Mouse
to treat atherosclerosis, and this process is characterized by excessive smooth muscle cell proliferation that re?occludes the vessel lumen. Aortic aneurysm formation is caused by severe degradation and thus dilatation of the vessel wall
【原创】转载Western Blot 内参选择攻略(美季生物技术有限公司)http://www.majorbio.cn/NewsInfo.asp?id=289
-tubulin。一般我们选择内参与要检测的目的蛋白的分子量最好相差5KD以上。因此你要知道你检测的蛋白的分子量来选择合适的内参! actin即肌动蛋白,是细胞的一种重要骨架蛋白。actin大致可分为六种,其中四种是不同肌肉组织特异性的,包括alpha-skeletal muscle actin、alpha-cardiac muscle actin、alpha-smooth muscle actin和gamma-smooth muscle actin,其余两种广泛分布于各种组织中,包括beta-actin(β
) Anti‐Pecam1 (V‐16, goat polyclonal, cat. no. SC‐31045) Anti‐smooth muscle actin (B4, mouse monoclonal, cat. no. SC‐53142
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