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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Neural cell adhesion molecule 2, Ncam2含量
- 适应物种:
小鼠
- 标记物:
Neural cell adhesion molecule 2, Ncam2
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Neural cell adhesion molecule 2, Ncam2 ELISA试剂盒
Product Name: Mouse Neural cell adhesion molecule 2, Ncam2 ELISA试剂盒
Product Name: Mouse Neural cell adhesion molecule 2, Ncam2 ELISA试剂盒
Gene Name: Mouse Neural cell adhesion molecule 2, Ncam2
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse Neural cell adhesion molecule 2, Ncam2
Alternative Name: Mouse Neural cell adhesion molecule 2, Ncam2
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse Neural cell adhesion molecule 2, Ncam2 ELISA试剂盒 allows for the in vitro quantitative determination of Mouse Neural cell adhesion molecule 2, Ncam2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse Neural cell adhesion molecule 2, Ncam2 ELISA试剂盒 has been pre-coated with an Mouse Neural cell adhesion molecule 2, Ncam2 antibody specific to Mouse Neural cell adhesion molecule 2, Ncam2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Neural cell adhesion molecule 2, Ncam2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Neural cell adhesion molecule 2, Ncam2 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Neural cell adhesion molecule 2, Ncam2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验神经细胞粘着分子(neural cell adhesion molecule,N-CAM)
N-CAM是膜糖蛋白,至少以三种形式存在,但都是由同一基因编码。其中两种是跨膜蛋白,第三种共价结合在细胞质膜的外表面。无论是何种存在方式,N-CAM分子都有一部分伸出到细胞外表面。三种N-CAM在细胞外的结构都一样,都含有与细胞粘着相关的结合位点。 将分离纯化的N-CAM加入到人工磷脂脂质体中, 这种人工脂质体能够相互粘着,但是加入了相应的抗体就不会发生粘着, 不加N-CAM的人工脂质体也不会发生粘着。由此可以推测:细胞粘着是由细胞质膜中的蛋白分子介导的,N
In Vitro Migration Assays of Neural Stem Cells
and environmental factors, such as the polysialylated form of neural cell adhesion molecule (NCAM) or chemokines such as CXCL12, in the directional migration of neural precursors.
Evaluation of Proliferation of Neural Stem Cells In Vitro and In Vivo
two basic protocols for the detection of the proliferation of neural stem cells (NSC). The first one addresses cell proliferation in cultures, starting with primary cell cultures isolated from the mouse subventricular zone (SVZ), in which SVZ?derived NSC
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