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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Beta-amyloid protein 42, Beta-APP42含量
- 适应物种:
小鼠
- 标记物:
Beta-amyloid protein 42, Beta-APP42
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Beta-amyloid protein 42, Beta-APP42 ELISA试剂盒
Product Name: Mouse Beta-amyloid protein 42, Beta-APP42 ELISA试剂盒
Product Name: Mouse Beta-amyloid protein 42, Beta-APP42 ELISA试剂盒
Gene Name: Mouse Beta-amyloid protein 42, Beta-APP42
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse Beta-amyloid protein 42, Beta-APP42
Alternative Name: Mouse Beta-amyloid protein 42, Beta-APP42
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse Beta-amyloid protein 42, Beta-APP42 ELISA试剂盒 allows for the in vitro quantitative determination of Mouse Beta-amyloid protein 42, Beta-APP42 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse Beta-amyloid protein 42, Beta-APP42 ELISA试剂盒 has been pre-coated with an Mouse Beta-amyloid protein 42, Beta-APP42 antibody specific to Mouse Beta-amyloid protein 42, Beta-APP42 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Beta-amyloid protein 42, Beta-APP42 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Beta-amyloid protein 42, Beta-APP42 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Beta-amyloid protein 42, Beta-APP42 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验[3]Zhang YW,Thompson R,Zhang H,et al.APP processing in Alzheimer's disease.Mol Brain,2011,4:3. [4]Sastre M,Steiner H,Fuchs K,et al.Presenilin-dependent gamma-secretes processing of beta-amyloid precursor protein at a site corresponding to the S3
Quantifying Aβ1–40 and Aβ1–42 Using Sandwich-ELISA
The role of Aβ accumulation in the pathogenesis of Alzheimer’s disease (AD) is supported by genetic studies showing that mutations in the amyloid-β precursor protein (APP) that alter Aβ production are linked to a subset of familial AD (FAD) cases
Membrane‐Based Yeast Two‐Hybrid System to Detect Protein Interactions
proteins) are relatively difficult to investigate by standard yeast two?hybrid methods either because they are retained at cellular membranes or they activate the system in the absence of a true protein interaction. The membrane?based yeast two?hybrid (MbY
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