Angiopoietin-4, Angpt4 ELISA K

Mouse Angiopoietin-4, Angpt4 ELISA试剂盒

Product Name: Mouse Angiopoietin-4, Angpt4 ELISA试剂盒

Product Name: Mouse Angiopoietin-4, Angpt4 ELISA试剂盒

Gene Name: Mouse Angiopoietin-4, Angpt4

Detect Range:78-5000pg/ml

Sensitivity:39pg/ml

Target Protein Name: Mouse Angiopoietin-4, Angpt4

Alternative Name: Mouse Angiopoietin-4, Angpt4
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description: Mouse Angiopoietin-4, Angpt4 ELISA试剂盒 allows for the in vitro quantitative determination of Mouse Angiopoietin-4, Angpt4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Angiopoietin-4, Angpt4 ELISA试剂盒 has been pre-coated with an Mouse Angiopoietin-4, Angpt4 antibody specific to Mouse Angiopoietin-4, Angpt4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Angiopoietin-4, Angpt4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Angiopoietin-4, Angpt4 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Angiopoietin-4, Angpt4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!