- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELA-E1342m
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白Alpha-Smooth muscle actin含量
- 适应物种：
小鼠
- 标记物：
Alpha-Smooth muscle actin
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Alpha-Smooth muscle actin ELISA试剂盒
Product Name: Mouse Alpha-Smooth muscle actin ELISA试剂盒
Product Name: Mouse Alpha-Smooth muscle actin ELISA试剂盒
Gene Name: Mouse Alpha-Smooth muscle actin
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse Alpha-Smooth muscle actin
Alternative Name: Mouse Alpha-Smooth muscle actin
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse Alpha-Smooth muscle actin ELISA试剂盒 allows for the in vitro quantitative determination of Mouse Alpha-Smooth muscle actin concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse Alpha-Smooth muscle actin ELISA试剂盒 has been pre-coated with an Mouse Alpha-Smooth muscle actin antibody specific to Mouse Alpha-Smooth muscle actin .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Alpha-Smooth muscle actin and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Alpha-Smooth muscle actin , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Alpha-Smooth muscle actin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验by reaction with alkaline phosphatase-conjugated goat anti-mouse secondary antibody. Choose the actin pool broadly, however, consider also checking the column fractions by dot blot with mAB 3F2 to identify capping protein. Pool the actin fractions
Isolation and Culture of Human Airway Smooth Muscle Cells
doublings and express smooth muscle cell-specific markers such as myosin heavy chain, myosin light chain kinase, α-actin, caldesmon, and SM22α . In addition, the synthetic function of these cells appears to be comparable to those studied in vivo
(3 ) and in vitro (4 –6 ). The pluripotent properties of ES cells are the basis of gene targeting technologies used to create mutant mouse strains with inactivated genes by homologous recombination (7 ).
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