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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Interleukin-1 beta, Il1b含量
- 适应物种:
小鼠
- 标记物:
Interleukin-1 beta, Il1b
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Interleukin- 1 beta, IL- 1b ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Interleukin-1 beta(IL-1 beta),;Catabolin,; interleukin-1 beta; IL-1beta; IL1beta; IL-1 beta; interleukin 1, beta
Search name
Mouse Interleukin-1 beta ELISA Kit, Mouse IL-1b ELISA Kit, Mouse Interleukin-1b ELISA Kit, Mouse IL-1beta ELISA Kit, Mouse
Catabolin ELISA KIT,Mouse IL1F2 ELISA KIT, Interleukin-1 beta ELISA Kit, IL-1b ELISA Kit, Interleukin-1b ELISA Kit, IL-1beta
ELISA Kit, Catabolin ELISA KIT, IL1F2 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse interleukin-1 beta,IL-1 beta concentrations in serum,
Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-1 beta. Standards or samples are then added
to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-1 beta and Avidin
conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to
each well. Only those wells that contain IL-1 beta, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color.
The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IL-1 beta in the samples is then determined by comparing
the O.D. of the samples to the standard curve.
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