Mouse IL1b(Interleukin 1 Beta) ELISA Kit
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Mouse IL1b(Interleukin 1 Beta)

ELISA Kit
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  • ¥1680 - 2400
  • ELK Biotechnology 科鹿生物已认证
  • 15.63-1000 pg/mL
  • 6.5 pg/mL
  • ELK1271
  • 2025年09月05日
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  • 企业认证

    • 详细信息
    • 文献和实验
    • 技术资料
    • 检测范围

      15.63-1000 pg/mL

    • 检测方法

      Sandwich

    • 灵敏度

      6.5 pg/mL

    • 规格

      48T/96T

    规格:48T产品价格:¥1680.0
    规格:96T产品价格:¥2400.0
    中文名称 小鼠白介素1β(IL1b)酶联免疫吸附检测试剂盒
    货号 ELK1271
    别称 IL-1B;IL1-B; IL1-Beta; IL1F2; IL-1β; Interleukin-1 Family Member 2; Catabolin
    试剂盒种类 常规法
    操作时间 3.5h
    样本体积 100μL
    可检测样本类型: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
    储存条件: 一、试剂盒拆封后可 4°C 保存 1 周,如果 1 周内未用完,请将剩余酶标板,冻干标准品,浓缩生物素化抗体,浓缩 HRP 酶结合物放置在-20°C 保存,其他试剂4°C 保存,并在6 个月内用完。
    二、若试剂盒未拆封:4°C(短时间存放,6 个月内有效);-20°C(长时间存放,1 年内有效);避免反复冻融,过期后请勿使用。
    三、 如果试剂盒整盒保存在-20°C,请在实验前一天晚上将试剂盒放置在4°C。
    试剂盒组成部分:
    中文名称 英文名称 规格 储存条件
    48T 96T
    酶标板(可拆) Pre-coated Microplate 6 条 x 8 孔 12 条 x 8 孔 4°C/-20°C
    冻干标准品 Standard (lyophilized) 1 2 4°C/-20°C
    标准品&样品稀释液 Standard/Sample Diluent Buffer 10 mL 20 mL 4°C/-20°C
    浓缩生物素化抗体(100×) Biotinylated Antibody (100×) 60 μL 120 μL 4°C/-20°C
    生物素化抗体稀释液 Biotinylated Antibody Diluent 6 mL 12 mL 4°C/-20°C
    浓缩HRP酶结合物(100×) Streptavidin-HRP (100×) 60 μL 120 μL 4°C/-20°C
    酶结合物稀释液 HRP Diluent 6 mL 12 mL 4°C/-20°C
    浓缩洗涤液(25×) Wash Buffer (25×) 10 mL 20 mL 4°C/-20°C
    显色底物溶液(TMB) TMB Substrate Solution 6 mL 10 mL 4°C/-20°C(避光)
    反应终止液 Stop Reagent 3 mL 6 mL 4°C/-20°C
    封板覆膜 Plate Covers 1 2 常温
    产品说明书 Instruction Manual 1份 1份 常温
    操作步骤:
    Mouse IL1b(Interleukin 1 Beta) 1. 试剂盒和样本室温平衡后,每孔加入标准工作缓冲液100 μL(按说明书梯度稀释)或样品100 μL, 37°C孵育80分钟。
    2. 弃去酶标板中液体,每孔加入200μL洗涤缓冲液,洗涤3次。 拍干后,每孔加入100μL 生物素化抗体工作液,37°C孵育50分钟。
    3. 弃去酶标板中液体,每孔加入200 μL洗涤缓冲液,洗涤3次。拍干后,每孔加入100 μL HRP酶工作液,37°C孵育50分钟。
    4. 弃去酶标板内液体,每孔加入200 μL洗涤缓冲液,洗涤5次。拍干后, 每孔加入90 μL TMB, 37℃孵育20 min 。
    5.每孔加入50 μL终止液,450 nm立即读数,计算结果。
    推荐标曲拟合信息: 见官网说明书详解
    微信扫码咨询客服:
    科鹿官方微信公众号:
    Mouse IL1b(Interleukin 1 Beta)       Mouse IL1b(Interleukin 1 Beta)

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    图标文献和实验
    该产品被引用文献
    • IF =3.7 Cucurbitacin B alleviates DSS-induced ulcerative colitis by improving gut microbiota disorder in C57BL/6 mice. AMB Express (2025)
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    • IF =5 The Administration of Circulating Extracellular Vesicles Modified by Anesthesia and Surgery Induces Delirium-Like Behaviors in Aged Mice. CNS Neuroscience & Therapeutics (2025)
    • IF =6.3 Isoferulic Acid Mitigates Acute Lung Injury Induced by Sepsis Through the Inhibition of JAK2. PHYTOTHERAPY RESEARCH (2025)
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    • IF =4.4 Xanthohumol ameliorates diabetic kidney disease through suppression of renal fibrosis by regulating SNHG10/miR-378b. Frontiers in Pharmacology (2025)
    • IF =3.5 Puerarin Delays the Progression of Muscle Atrophy in Mice With Dexamethasone-Induced Sarcopenia Through Inhibiting the TNF-α/NF-κB Pathway. Food Science & Nutrition (2025)
    • IF =3.4 Abnormal lipid metabolism and inflammatory response induced by aluminum led to the cognitive decline in mice. NEUROTOXICOLOGY (2025)
    • IF =3.4 Integrated bioinformatics and experiment validation reveal cuproptosis-related biomarkers and therapeutic targets in sepsis-induced myocardial dysfunction. BMC Infectious Diseases (2025)
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    • IF =12.8 Modification of MSCs with aHSCs-Targeting Peptide pPB for Enhanced Therapeutic Efficacy in Liver Fibrosis. BIOMATERIALS (2025)
    • IF =4.5 Puerarin Ameliorates Sarcopenia in Aged Mice via Modulation of Inflammation and Oxidative Stress: Insights from Proteomics and Transcriptomics. Inflammation (2025)
    • IF =4.4 Thonningianin A ameliorates acetaminophen-induced liver injury by activating GPX4 and modulating endoplasmic reticulum stress. Frontiers in Pharmacology (2025)
    • IF =3.1 cGAS/STING/NLRP3 Signaling Pathway-Mediated Pyroptosis in Hypertrophic Cardiomyopathy Radiotherapy. Frontiers in Bioscience-Landmark (2025)
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    • IF =5.7 Indole-3-Propionic Acid Attenuates Neuroinflammation and Cognitive Deficits by Inhibiting the RAGE-JAK2-STAT3 Signaling Pathway. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2025)
    • IF =5 Inflammation induced PFKFB3-mediated glycolysis promoting myometrium contraction through the PI3K-Akt-mTOR pathway in preterm birth mice. AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY (2025)
    • IF =3.6 E3 Ubiquitin Ligase TRIM7 Alleviates Lipopolysaccharide-Induced Acute Lung Injury via Inhibiting NLRP3 Inflammasome Activation. The American Journal of Pathology (2025)
    • IF =10 Glucose-Responsive Zn(II)-Porphyrin COF Adhesive Hydrogels With Dual-Active Sites and GOX-Like Activity for Accelerated Wound Healing. Advanced Healthcare Materials (2025)
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    • IF =14.3 Engineered Macrophage Membrane-Coated S100A9-siRNA for Ameliorating Myocardial Ischemia-Reperfusion Injury. Advanced Science (2024)
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    • IF =3.4 Aluminum-induced oxidative stress promotes changes in the structure of the gut microbiota and liver deficiency. Heliyon (2024)
    • IF =5.7 Fisetin Promotes Functional Recovery after Spinal Cord Injury by Inhibiting Microglia/Macrophage M1 Polarization and JAK2/STAT3 Signaling Pathway. Journal of Agricultural and Food Chemistry (2024)
    • IF =8.3 Inhibition of Transmural Inflammation in Crohn’s Disease by Orally Administered Tumor Necrosis Factor-Alpha Deoxyribozymes-Loaded Pyroptosis Nanoinhibitors. ACS Applied Materials & Interfaces (2024)
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    • IF =4 Extracellular vesicles containing miR-181a-5p as a novel therapy for experimental autoimmune encephalomyelitis-induced demyelination. International Immunopharmacology (2024)
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    • IF =2.9 Macrophage-Targeted Versus Free Calcitriol as Host-Directed Adjunct Therapy Against Mycobacterium Tuberculosis Infection in Mice is Bacteriostatic and Mitigates Tissue Pathology. Tuberculosis (2024)
    • IF =8.2 MG53/GMs/HA-Dex neural scaffold promotes the functional recovery of spinal cord injury by alleviating neuroinflammation. International Journal of Biological Macromolecules (2024)
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    • IF =7.5 Shen Qi Wan ameliorates nephritis in chronic kidney disease via AQP1 and DEFB1 regulation. Biomedicine & Pharmacotherapy (2023)
    • IF =3.4 Caveolin-1 deficiency alleviates palmitate-induced intracellular lipid accumulation and inflammation in pancreatic β cells. Journal of Physiology and Biochemistry (2023)
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    • IF =3.6 Poricoic acid A induces mitophagy to ameliorate podocyte injury in diabetic kidney disease via downregulating FUNDC1. JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2023)
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    • IF =2 Dexmedetomidine attenuates neuroinflammation and microglia activation in LPs-stimulated bV2 microglia cells through targeting circ-shank3/mir-140-3p/tLr4 axis. EUROPEAN JOURNAL OF HISTOCHEMISTRY (2023)
    • IF =2 Dexmedetomidine attenuates neuroinflammation and microglia activation in LPS-stimulated BV2 microglia cells through targeting circ-Shank3/mir-140-3p/TLR4 axis. EUROPEAN JOURNAL OF HISTOCHEMISTRY (2023)
    • IF =5.62 Exosomal miR-23b-3p from bone mesenchymal stem cells alleviates experimental autoimmune encephalomyelitis by inhibiting microglial pyroptosis. Experimental Neurology (2023)
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    • IF =2.65 Dl-3-n-Butylphthalide (NBP) Mitigates Muscular Injury Induced by Limb Ischemia/Reperfusion in Mice through the HMGB1/TLR4/NF-κB Pathway. Evidence-Based Complementary and Alternative Medicine (2022)
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    • IF =2.629 Sulforaphane Ameliorates Limb Ischemia/Reperfusion-Induced Muscular Injury in Mice by Inhibiting Pyroptosis and Autophagy via the Nrf2-ARE Pathway. Evidence-based Complementary and Alternative Medicine (2022)
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    • IF =15.153 Cell-Free Extracts from Human Fat Tissue with a Hyaluronan-Based Hydrogel Attenuate Inflammation in a Spinal Cord Injury Model through M2 Microglia/Microphage Polarization. Small (2022)
    • IF =2.447 Se alleviates homocysteine‑induced fibrosis in cardiac fibroblasts via downregulation of lncRNA MEG3. Experimental and Therapeutic Medicine (2021)
    • IF =3.612 Methylation of miR‐19b‐3p promoter exacerbates inflammatory responses in sepsis‐induced ALI via targeting KLF7. CELL BIOLOGY INTERNATIONAL (2021)
    • IF =4.799 Subconjunctival Injection of Regulatory T Cells Potentiates Corneal Healing Via Orchestrating Inflammation and Tissue Repair After Acute Alkali Burn. Investigative Ophthalmology & Visual Science (2020)
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    • IF =3.211 Rho kinase blockade ameliorates DSS-induced ulcerative colitis in mice through dual inhibition of the NF-κB and IL-6/STAT3 pathways. INFLAMMATION (2020)
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    文献支持
    Mouse IL1b(Interleukin 1 Beta) ELISA Kit
    ¥1680 - 2400