- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- CELI-66117m
- 2026年03月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
10
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白Cyclic guanosine monophosphate, CGMP含量
- 适应物种：
小鼠
- 标记物：
Cyclic guanosine monophosphate, CGMP
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Cyclic guanosine monophosphate, cGMP ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Cyclic guanosine monophosphate,;
Search name
Mouse Cyclic guanosine monophosphate ELISA Kit, Mouse cGMP ELISA Kit, Cyclic guanosine monophosphate ELISA Kit,
cGMP ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of cyclic guanosine monophosphate,Cyclic guanosine
monophosphate concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Cyclic guanosine monophosphate, During the reaction, Cyclic guanosine monophosphate in the
sample or standard competes with a fixed amount of biotin-labeled Cyclic guanosine monophosphate for sites on a pre-coated
Monoclonal antibody specific to Cyclic guanosine monophosphate. Excess conjugate and unbound sample or standard are washed from
the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB
substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the
color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Cyclic guanosine
monophosphate in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF manual download
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文献和实验Of the 11 families of mammalian cyclic nucleotide phosphodiesterases (PDEs), 5 contain regulatory domains capable of binding cyclic guanosine 5′-monophosphate (cGMP). The best understood of the GAF-containing PDEs is the family of rod (PDE
Detection of cGMP in the Degenerating Retina
Cyclic guanosine 3′-5′-monophosphate (cGMP) plays a key role in the physiological process of light detection in photoreceptor cells of the retina. However, there is also growing evidence that cGMP may be critically involved
Cygnets: In Vivo Characterization of Novel cGMP Indicators and In Vivo Imaging of Intracellular cGMP
The second messenger cyclic guanosine 5′-monophosphate (cGMP) plays a key role in the control and regulation of a steadily increasing number of diverse physiological processes. As the appreciation of the importance of understanding the cGMP
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