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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Cyclic adenosine monophosphate, cAMP含量
- 适应物种:
小鼠
- 标记物:
Cyclic adenosine monophosphate, cAMP
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Cyclic adenosine monophosphate, cAMP ELISA试剂盒
Product Name: Mouse Cyclic adenosine monophosphate, cAMP ELISA试剂盒
Product Name: Mouse Cyclic adenosine monophosphate, cAMP ELISA试剂盒
Gene Name: Mouse Cyclic adenosine monophosphate, cAMP
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse Cyclic adenosine monophosphate, cAMP
Alternative Name: Mouse Cyclic adenosine monophosphate, cAMP
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse Cyclic adenosine monophosphate, cAMP ELISA试剂盒 allows for the in vitro quantitative determination of Mouse Cyclic adenosine monophosphate, cAMP concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse Cyclic adenosine monophosphate, cAMP ELISA试剂盒 has been pre-coated with an Mouse Cyclic adenosine monophosphate, cAMP antibody specific to Mouse Cyclic adenosine monophosphate, cAMP .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Cyclic adenosine monophosphate, cAMP and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Cyclic adenosine monophosphate, cAMP , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Cyclic adenosine monophosphate, cAMP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Study of Cyclic Adenosine Monophosphate Microdomains in Cells
Cyclic adenosine monophosphate (cAMP) controls the physiological response to many diverse extracellular stimuli. To maintain signal specificity, cAMP-mediated signaling is finely tuned by means of a complex array of proteins that control
High-Resolution Measurements of Cyclic Adenosine Monophosphate Signals in 3D Microdomains
A large number of hormones, neurotransmitters, and odorants exert their effects on cells by triggering changes in intracellular levels of cyclic adenosine monophosphate (cAMP). Although the effector proteins that bind cAMP
Extraction and Assay of Cyclic Nucleotides
Cyclic adenosine 3′,5′-monophosphate (cAMP) and cyclic guanosine 3′,5′-monophosphate (cGMP) have between them been implicated in the mediation of many physiological phenomena. Over the last 30 years, a number of techniques have been employed
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