The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
1:500 - 1:2000
IHC
1:100 - 1:200
Western blot analysis of extracts of various cell lines, using MMP9 antibody.
Immunohistochemistry of paraffin-embedded mouse heart using MMP9 antibody.
Immunohistochemistry of paraffin-embedded human liver injury using MMP9 antibody.
Immunohistochemistry of paraffin-embedded mouse liver using MMP9 antibody.
Immunohistochemistry of paraffin-embedded mouse kidney using MMP9 antibody.
Background:
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.
Storage:
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.