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- LSM BIO
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- PVT14605
- 2025年07月15日
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- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pET15b-Halo-TB-10-E
- 库存：
50
- 供应商：
LSMBIO
- 规格：
2ug
pET15b-Halo-TB-10-E/ pET15b-Halo-TB-10-E璐ㄧ矑
pET15b-Halo-TB-10-E
PVT14605 2ug
pET15b-Halo-TB-10-E Search name
pET15b-Halo-TB-10-E,pET15b-Halo-TB-10-E vector,pET15b-Halo-TB-10-E plasmid,pET15b-Halo-TB-10-E map
pET15b-Halo-TB-10-E Information
Bacterial Resistance(s): Amp
Screening:
Growth Strain(s): DH5 alpha
Growth Condition: LB/37 Centigrade
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文献和实验Purification of Plasmid from 50 ml-culture
1. Shake E. coli harboring plasmid at 37 C overnight in 50 ml of TB containing appropriate antibiotics. (when using ampicillin, addition of the antibiotics to 100-200 ug/ml rather than usual 50 ug/ml may improve the yield of plasmids.)
This protocol is for Mini (up to 20 µg) preparations of high-copy plasmid DNA from cultures of E. coli . Important notes before starting New users are strongly recommended to read Appendix A (page 21) at the end
isopropanol and mixed.Spin 10-15 min.Discard supernatant and wash pellet with 1 ml of 100% EtOH.dry pellets and resuspend in 50 µl H2O or TE.Buffer A:50 mM TrisHCl pH 8.0,10 mM EDTA,100 ug/ml RNase A. Buffer B:200 mM NaOH,1% SDS. 7.5 M NH4Ac (57.75
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