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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
负20摄氏度
- 保质期:
3个月
- 英文名:
lenti sgRNA (MS2)_puro backbone
- 库存:
50
- 供应商:
LSMBIO
- 规格:
2ug
lenti sgRNA (MS2)_puro backbone/ lenti sgRNA (MS2)_puro backbone璐ㄧ矑
lenti sgRNA(MS2)_puro backbone
Catalog No. PVT11345
Packing 2ug
Function /
Resistance /
Screen /
Strain /
Culture temperature
Replicon
Copy
Promoter
Induction
Forward primer
Reverse primer
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文献和实验reaction. Mix the same reagents without addingthe sgRNA oligo duplex to prepare a negative control reaction. Incubate the ligation reaction in a thermocycler followingthe parameters below. 3. (Optional but highly recommended) Plasmid-Safe
Construction of Gene-Targeting Vectors by Recombineering
DNA to a Plasmid Backbone (2 d) 24. Inoculate one BsdR -KanR -AmpS -HygroR colony into 1.0 mL of LB with Kan in a 15-mL polypropylene tube and incubate the tube overnight at 32°C with shaking at 200 rpm. 25. Inoculate
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