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- PVT10370
- 2025年07月12日
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- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pCDNA3.1-RHEB-Q60K-Myc
- 库存：
50
- 供应商：
LSMBIO
- 规格：
2ug
pCDNA3.1-RHEB-Q60K-Myc/ pCDNA3.1-RHEB-Q60K-Myc璐ㄧ矑
pCDNA3.1-RHEB-Q60K-Myc
Catalog No. PVT10370
Packing 2ug
Function ORF expression plasmid
Resistance Amp
Screen Neo/G418
Strain DH5alpha
Culture temperature 37degrees centigrade
Replicon PUC
Copy high
Promoter CMV
Induction --
Forward primer CMV-F
Reverse primer
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文献和实验the cells by spinning in a 500 ml bottle in the J6B at 4.2K for 20 minutes.Resuspend the pellet gently in 30 ml Solution I with 1 mg/ml lysozyme(Sigma #L6876). 3、Transfer immediately to a 250 ml bottle on ice and add 60 ml Solution II,followed by 45 ml
This protocol is for Mini (up to 20 µg) preparations of high-copy plasmid DNA from cultures of E. coli . Important notes before starting New users are strongly recommended to read Appendix A (page 21) at the end
Plasmid Profile Typing and Plasmid Fingerprinting
Plasmids are extra-chromosomal molecules of deoxyribonucleic acid (DNA) capable of autonomous replication. Such molecules have been identified in many bacterial genera and usually exist as covalently closed circular (CCC) molecules. Plasmids
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