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- LSM BIO
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- PVT10355
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
DsRed-rab7 WT
- 库存：
50
- 供应商：
LSMBIO
- 规格：
2ug
DsRed-rab7 WT/ DsRed-rab7 WT璐ㄧ矑
DsRed-rab7 WT
Catalog No. PVT10355
Packing 2ug
Function ORF expression plasmid
Resistance Kan
Screen Neo/G418
Strain DH5alpha
Culture temperature 37degrees centigrade
Replicon
Copy
Promoter
Induction
Forward primer
Reverse primer
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文献和实验Enzyme Kinetics assay of the WT
* The day before transfection, split and plating the cells, so the that they are 50%- 60% confluent the day of transfection. Avoid antibiotics at the time of transfection and during. * Culture vessel:100mm * plasmid DNA ; 4 microg
Enzyme Kinetics assay of the WT
Enzyme Kinetics assay of the WT To assay 17 b-HSD activity in lysates, cells were harvested 48h after transfection using PBS Enzyme Free Cell Dissociation Solution ( specialty Media Inc., Lavellette, NJ), frozen on dry ice and stored
Preparation of Plasmid DNA Using Alkaline Lysis
precipitation of the bacterial chromosomal DNA and other high-mol-wt cellular structures. The plasmid DNA, which stays in suspension, is then precipitated with isopropanol.
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