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- 文献和实验
- 技术资料
- 规格:
100 µL
抗体种属:Human
抗体靶点:IFNL3
抗体应用:ELISA
抗原货号:11890-H08H
抗原描述:Recombinant Human IL-28B/IFN-lambda-3 Protein (Catalog#11890-H08H)
抗体宿主:Mouse
抗体Ig类型:Mouse IgG2b
抗体纯化方法:Protein A
抗体制备:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IL-28B/IFN-lambda-3 (rh IL-28B/IFN-lambda-3; Catalog#11890-H08H; NP_742151.2; Met1-Val196). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
抗体特异性:Human IL-28B/IFN-lambda-3
抗体保存:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
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文献和实验的培养及鉴定 2.1 将PBMC按1-2 x 106/ml的浓度悬浮于无血清培养液中,加入1,000 U/ml 的重组人IFN-g,37oC,5%CO2培养箱中培养; 2.2 24h 后加入50ng/ml 的CD3 单克隆抗体和300 U/ml 的重组人IL-2,刺激CIK 细胞的生长和增殖; 注:此时也可同时加入100 U/ml的重组人IL-1a。 2.3 每3天半量换液或扩瓶一次,并补加重组人IL-2 300 U/ml; 2.4 在培养的第14d,收获CIK细胞。
Dual-Color ELISPOT Assay for the Simultaneous Detection of IL-2 and/or IFN- Secreting T Cells
because of the known antiviral effects of this cytokine as well as its correlation to the cytotoxic capacity of T cells. However, maintenance of both IFN- and IL-2 secretion by pathogen-specific T cells has been linked to a more favorable clinical outcome in human
朱伟民,与著名学者余国良等共同创办 Epitomics 公司(专利创新技术:RabMAb® ,兔单抗开发平台拥有者)。朱伟民于厦门大学获得细胞生物学硕士学位(M.S.),随后在浙江大学病理研究所进行鼠单抗开发工作,之后在意大利米兰大学药理学院做访问学者。朱伟民老师曾任职于 UCSF Gladstone Institutes 和 Lung Biology Center,在 UCSF 期间发明 RabMAb® 技术,共发表文献 20 多篇,拥有共同发明专利 10 多个,且曾在加州大学深造并获得
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