pLL3.7m-mTurquoise2-SLBP(18-126)-IRES-H1-mMaroon1
PVT10347 2ug
pLL3.7m-mTurquoise2-SLBP(18-126)-IRES-H1-mMaroon1 Sequence
LOCUS Exported 9431 bp ds-DNA circular SYN 27-SEP-2017
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9431)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 27, 2017
FEATURES Location/Qualifiers
source 1..9431
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 101..562
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
CDS 556..1902
/codon_start=1
/label=H1-mMaroon1
/translation="MIMATEFMTENSTSAPAAKPKRAKASKKSTDHPKYSDMIVAAIQA
EKNRAGSSRQSIQKYIKSHYKVGENADSQIKLSIKRLVTTGVLKQTKGVGASGSFRLAK
GDEPKRSVAFKKTKKEVKKVATPKKAAKPKKAASKAPSKKPKATPVKKAKKKPAATPKK
AKKPKVVKVKPVKASKPKKAKTVKPKAKSSAKRGSKKKSGLRSGGSGGMVSKGEELIKE
NMHTKLYLTGTVNNHYFECTAEGEGKPYEGTQTNRIKVVRGGPLPFAFDILAPCFMYGS
KTFINHPPDIPDYFKQSFPEGFTWERTTVYEDGGTLTATQDTSLQDGCLIYNVQVRGEN
FPSNGPVMQKKTLGWEASTETLYPADGSLEGRLDWALKLVGGGHLHCRLETTYRSKKPA
KNLKMPGVYFIDRRLERIKEADNETYVEQHEVAVARYCDLPSKLGHKLN"
protein_bind 1930..1963
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
misc_feature 2019..2607
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
LTR 2849..3029
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
rep_origin complement(3091..3679)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3850..4710)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4711..4815)
/gene="bla"
/label=AmpR promoter
enhancer 4906..5209
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 5211..5409
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
LTR 5427..5607
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 5654..5779
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 6276..6509
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 7036..7153
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
protein_bind 7599..7632
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
enhancer 7748..8051
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 8052..8255
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 8291..9007
/codon_start=1
/product="enhanced monomeric variant of CFP (Goedhart et
al., 2012)"
/label=mTurquoise2
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVL <