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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
负20摄氏度
- 保质期:
3个月
- 英文名:
pCD513B-1-CD123
- 库存:
100
- 供应商:
LSMBIO
- 规格:
2ug
pCD513B-1-CD123 Plasmid
Catalog No. PVTB00716-4a
Vector Backbone pCDH-CMV-MCS-EF1-CopGFP-T2A-Puro
Backbone manufacturer Clontech
Vector type Mammalian Expression, Lentiviral
Backbone size w/o insert 8189bp
Cloning site 5 Xba I
Site destroyed during cloning NO
Cloning site 3 Not I
Bacterial resistance Ampicillin
Growth strain Stbl3
Growth temperature 37 Centigrade
High or low copy High Copy
Selectable markers Puromycin
Background Lentiviral expression vector of Homo sapiens interleukin 3 receptor subunit alpha with the unfused CopGFP protein at the C-terminus
Gene Information
Alternative Gene Name IL3R; IL3RA; IL3RX; IL3RY; IL3RAY; hIL-3Ra
Insert Gene name CD123
Insert size 1137bp
Accessions BC035407
Tag CopGFP(C-term)
Species Homo sapiens (human)
Gene ID 3563
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Fluorogenic Substrates as Detectors of Caspase Activity During Natural Killer Cell-Induced Apoptosis
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温度越来越低[2 ] ,从而达到最佳扩增条件的方法。我们在构建人 CD137 - hIgG1Fc - pCDNA3 融合蛋白真核表达载体以及在检测HBV DNA 多聚酶基因突变的过程中运用降落PCR 进行了扩增目的基因的尝试。1 材料和方法1.1 质粒含有人CD137 全长cDNA 序列的pCDNA3 质粒(CD137 - pCDNA3) ,由Herbert Schwarz 教授惠赠; 含hIgG1 FccDNA的PGEM - T质粒由第二军医大学曹雪涛教授惠赠; HBV DNA ,从6 位慢性HBV 感染者血清
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