- ￥1325
- LSM BIO
- 进口
- PVT3395
- 2026年03月16日
8 年钻石会员
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pSET152 vector
- 库存：
10
- 供应商：
LSMBIO
- 规格：
质粒干粉
pSET152 vector
Packing 2ug
Alternative name
pSET152 vector,pSET152, vector pSET152, pSET152 Plasmid, Plasmid pSET152
pSET152 Information
Promoter: No, it needs to be cloned by itself
Prokaryotic Apramycin resistance: Apr
Screening marker: Apramycin Apr
Cloned strain: Escherichia coli DH5 alpha
Culture conditions: 30 degrees centigrade
Expression host: Streptomyces, actinomycetes, etc.
pSET152 Description
PSET152 is a integrated expression plasmid of Streptomyces. PSET152 vector can integrate exogenous genes into Streptomyces genome, and is a commonly used gene delivery vector for Streptomyces genome to express highly specific genes.
pSET152 Sequence
LOCUS Exported 5715 bp ds-DNA circular SYN 27-JAN-2018
FEATURES Location/Qualifiers
source 1..5715
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS complement(163..507)
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/label=lacZ-alpha
/translation="MITNSISRAAADPLESTCSPSLALAVVLQRRDWENPGVTQLNRLA
AHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRINSPMSSTSGIG
SAADAKCRST"
rep_origin complement(910..1498)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS 1702..2502
/codon_start=1
/gene="aac(3)IV"
/product="aminoglycoside 3-N-acetyltransferase type IV
"
/label=Apr
/note="confers resistance to apramycin (Paget and Davies,
1996)"
/translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
CDS complement(2936..3307)
/codon_start=1
/gene="traJ"
/product="oriT-recognizing protein"
/label=traJ
/translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
KQDELGKVMMGVVRPRAEP"
oriT 3340..3449
/note="incP origin of transfer"
protein_bind 3769..3868
/label=phage phi-C31 attP
/bound_moiety="phage phi-C31 integrase"
/note="attachment site of phage phi-C31"
CDS 3878..5695
/codon_start=1
/product="integrase from phage phi-C31"
/label=phage phi-C31 integrase
/translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVP
IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
EKRASITWAKPPTDDDEDDAQDGTEDVAA"
ORIGIN
1 atctacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca
61 gctctcgcag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt
121 cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttg atcggcactt
181 tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattta tgcggtgtga
241 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct
301 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
361 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
421 ttgtaaaacg acggccagtg ccaagcttgg gctgcaggtc gactctagag gatccgcggc
481 cgcgcgcgat atcgaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc
541 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
601 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
661 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
721 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
781 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
841 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
901 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
961 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
1021 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
1081 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
1141 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
1201 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
1261 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
1321 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
1381 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
1441 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
1501 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
1561 gattttggtc atgagattat caaaaaggat cttcacctag atccttttgg ttcatgtgca
1621 gctccatcag caaaagggga tgataagttt atcaccaccg actatttgca acagtgccgt
1681 tgatcgtgct atgatcgact gatgtcatca gcggtggagt gcaatgtcgt gcaatacgaa
1741 tggcgaaaag ccgagctcat cggtcagctt ctcaaccttg gggttacccc cggcggtgtg
1801 ctgctggtcc acagctcctt ccgtagcgtc cggcccctcg aagatgggcc acttggactg
1861 atcgaggccc tgcgtgctgc gctgggtccg ggagggacgc tcgtcatgcc ctcgtggtca
1921 ggtctggacg acgagccgtt cgatcctgcc acgtcgcccg ttacaccgga ccttggagtt
1981 gtctctgaca cattctggcg cctgccaaat gtaaagcgca gcgcccatcc atttgccttt
2041 gcggcagcgg ggccacaggc agagcagatc atctctgatc cattgcccct gccacctcac
2101 tcgcctgcaa gcccggtcgc ccgtgtccat gaactcgatg ggcaggtact tctcctcggc
2161 gtgggacacg atgccaacac gacgctgcat cttgccgagt tgatggcaaa ggttccctat
2221 ggggtgccga gacactgcac cattcttcag gatggcaagt tggtacgcgt cgattatctc
2281 gagaatgacc actgctgtga gcgctttgcc ttggcggaca ggtggctcaa ggagaagagc
2341 cttcagaagg aaggtccagt cggtcatgcc tttgctcggt tgatccgctc ccgcgacatt
2401 gtggcgacag ccctgggtca actgg
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验该产品被引用文献
Structural basis for the interaction of the chaperone Cbp3 with newly synthesized cytochrome b during mitochondrial respiratory chain assembly
Mama Ndi1, Geoffrey Masuyer1, Hannah Dawitz1, Andreas Carlstr?m1, Mirco Michel1, Arne Elofsson2, Mikaela Rapp1, P?l Stenmark3 and Martin Ott3*
First Published on Journal of biological chemistry
September 19, 2019
doi: 10.1074/jbc.RA119.010483
jbc.RA119.010483.
相关实验
Direct Cloning of Agtll cDNA Inserts Into a Plasmid Vector
during the subcloning steps. Both polymerase chain reaction (PCR) (3 ,4 ) and plasmid rescue using the f 1 origin of replication, as in the λZAP systems (5 ), were developed to circumvent this problem. However, these sophisticated procedures may not exist
Loss-of-Function Studies in Mouse Embryonic Stem Cells Using the pHYPER shRNA Plasmid Vector
RNA interference is widely used for loss-of-function studies in mammalian cells. As an alternative to the transfection of small RNAs, plasmid vectors have been developed to express short hairpin RNAs (shRNAs). We engineered the pHYPER shRNA
Purpose: The method is used to clone smaller portions of inserts (up to 15 kb in size)which previously have been cloned in YACs phage cosmids or other plasmids. Time required: Plasmid Vector Preparation Restriction digest and Calf Intestinal
技术资料暂无技术资料 索取技术资料
文献支持
pSET152 vector Plasmid
￥1325
