- ￥1080
- LSM Bio
- 进口
- PVT5402
- 2025年07月13日
7 年钻石会员
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pPD95-67
- 库存：
100
- 供应商：
LSMBIO
- 规格：
2ug
PPD95_67
Search name
pPD95-67,Plasmid pPD95-67,pPD95-67 vector
PPD95_67 Informaiton
Replicon: pUC ori
Plasmid classification: nematode expression plasmid
Plasmid size: 4532bp
Plasmid tagging: IVSA, SV40NLS, gfpN, IVSR, gfpS65C, IVSS, IVST, gfpC, IVSL, decoy
Prokaryotic resistance: ampicillin Ampicillin
Clonal strain: DH5 alpha
Culture conditions: 37 C, aerobic, LB
Expression host: nematode
Culture conditions: refer to relevant literature.
Ways of induction: referring to relevant literature
5'sequencing primers: M13R:CAGGAAACAGCTATGACC
3'sequencing primers: Based on full sequence design
PPD95_67 Description
PPD95_67 is a nematode expression plasmid with GFP tag.
PPD95_67 Multiple cloning site
PPD95_67 Sequence
LOCUS Exported 4532 bp ds-DNA circular SYN 04-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pPD95_67
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4532)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sunday, September 4, 2016 from SnapGene Viewer 3.1.4
FEATURES Location/Qualifiers
source 1..4532
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 146..166
/codon_start=1
/product="nuclear localization signal of SV40 large T
antigen"
/note="SV40 NLS"
/translation="PKKKRKV"
misc_feature 167..580
/note="GFP"
promoter 2277..2381
/gene="bla"
/note="AmpR promoter"
CDS 2382..3242
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 3413..4001
/direction=RIGHT
/note="pUC ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 4289..4310
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 4325..4355
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 4363..4379
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 4387..4403
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gattggccaa aggacccaaa
61 ggtatgtttc gaatgatact aacataacat agaacatttt caggaggacc cttggagggt
121 accgagctca gaaaaaatga ctgctccaaa gaagaagcgt aaggtaccgg tagaaaaaat
181 gagtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga
241 tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa
301 acttaccctt aaatttattt gcactactgg aaaactacct gttccatggg taagtttaaa
361 catatatata ctaactaacc ctgattattt aaattttcag ccaacacttg tcactacttt
421 ctgttatggt gttcaatgct tctcgagata cccagatcat atgaaacggc atgacttttt
481 caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg
541 gaactacaag acacgtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt
601 ttcaggtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg
661 tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc
721 acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagttg taagtttaaa
781 catgatttta ctaactaact aatctgattt aaattttcag aacttcaaaa ttagacacaa
841 cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga
901 tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga
961 tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac
1021 acatggcatg gatgaactat acaaatagca ttcgtagaat tccaactgag cgccggtcgc
1081 taccattacc aacttgtctg gtgtcaaaaa taataggggc cgctgtcatc agagtaagtt
1141 taaactgagt tctactaact aacgagtaat atttaaattt tcagcatctc gcgcccgtgc
1201 ctctgacttc taagtccaat tactcttcaa catccctaca tgctctttct ccctgtgctc
1261 ccacccccta tttttgttat tatcaaaaaa cttctcttaa tttctttgtt ttttagcttc
1321 ttttaagtca cctctaacaa tgaaattgtg tagattcaaa aatagaatta attcgtaata
1381 aaaagtcgaa aaaaattgtg ctccctcccc ccattaataa taattctatc ccaaaatcta
1441 cacaatgttc tgtgtacact tcttatgttt tttacttctg ataaattttt ttgaaacatc
1501 atagaaaaaa ccgcacacaa aataccttat catatgttac gtttcagttt atgaccgcaa
1561 tttttatttc ttcgcacgtc tgggcctctc atgacgtcaa atcatgctca tcgtgaaaaa
1621 gttttggagt atttttggaa tttttcaatc aagtgaaagt ttatgaaatt aattttcctg
1681 cttttgcttt ttggggtttc ccctattgtt tgtcaagatt tcgaggacgg cgtttttctt
1741 gctaaaatca caagtattga tgagcacgat gcaagaaaga tcggaagaag gtttgggttt
1801 gaggctcagt ggaaggtgag tagaagttga taatttgaaa gtggagtagt gtctatgggg
1861 tttttgcctt aaatgacaga atacattccc aatataccaa acataactgt ttcctactag
1921 tcggccgtac gggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
1981 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
2041 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
2101 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
2161 gagaaaatac cgcatcaggc ggccttaagg gcctcgtgat acgcctattt ttataggtta
2221 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
2281 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
2341 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
2401 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
2461 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc <
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验该产品被引用文献
Upregulation of Antioxidant Capacity and Nucleotide Precursor Availability Suffices for Oncogenic Transformation
Author：YangZhang1YiXu1WenyunLu23Jonathan M.Ghergurovich24LiliGuo56Ian A.Blair5Joshua D.Rabinowitz23XiaoluYang17
Received 22 January 2020, Revised 4 August 2020, Accepted 30 September 2020, Available online 6 November 2020.
Published: November 6, 2020
Cell Metabolism
Available online 6 November 2020 In Press
doi: doi.org/10.1016/j.cmet.2020.10.002
技术资料暂无技术资料 索取技术资料
文献支持
pPD95-67
￥1080
