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- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pGADT7-Rec
- 库存：
100
- 供应商：
LSMBIO
- 规格：
2ug
pGADT7-Rec
Search name
pGADT7-Rec,Plasmid pGADT7-Rec,pGADT7-Rec vector
pGADT7-Rec Plasmid information
Promoter: T7, ADH1 promoter
Replicator: 2 ori, ori
Plasmid classification: yeast series, yeast single hybrid carrier
Plasmid size: 8058bp
Prokaryotic resistance: ampicillin Amp
Screening markers: LEU2
Cloned strains of Escherichia coli, DH5 A and other Escherichia coli
Culture conditions: 37 centigrade, aerobic, LB
Expression host: yeast cells
5'sequencing primers: T7:TAATACGACTCACTATAGGG
3'sequencing primers: primers designed according to sequence
Use: Yeast expression
pGADT7-Rec Plasmid Sequence
LOCUS Exported 8058 bp ds-DNA circular SYN 10-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled 22
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8058)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..8058
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 4..1168
/note="2u ori"
/note="yeast 2u plasmid origin of replication"
promoter 1450..1554
/gene="bla"
/note="AmpR promoter"
CDS 1555..2415
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 2586..3174
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 3414..3447
/bound_moiety="Cre recombinase"
/note="loxP"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
protein_bind 3525..3558
/bound_moiety="Cre recombinase"
/note="loxP"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
protein_bind 3613..3634
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 3649..3679
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 3687..3703
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 3711..3727
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
promoter 3769..4174
/gene="S. cerevisiae LEU2"
/note="LEU2 promoter"
CDS 4175..5269
/codon_start=1
/gene="S. cerevisiae LEU2"
/product="3-isopropylmalate dehydrogenase, required for
leucine biosynthesis"
/note="LEU2"
/note="yeast auxotrophic marker"
/translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
IGGAAIEATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
DLGGSNSTTEVGDAVAEEVKKILA"
terminator 5386..5573
/gene="S. cerevisiae ADH1"
/note="ADH1 terminator"
/note="transcription terminator for the S. cerevisiae
alcohol dehydrogenase 1 (ADH1) gene"
CDS complement(6092..6118)
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/note="HA"
/translation="YPYDVPDYA"
promoter complement(6137..6155)
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
CDS complement(6161..6502)
/codon_start=1
/gene="Saccharomyces cerevisiae GAL4 (truncated)"
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文献和实验该产品被引用文献
Upregulation of Antioxidant Capacity and Nucleotide Precursor Availability Suffices for Oncogenic Transformation
Author：YangZhang1YiXu1WenyunLu23Jonathan M.Ghergurovich24LiliGuo56Ian A.Blair5Joshua D.Rabinowitz23XiaoluYang17
Received 22 January 2020, Revised 4 August 2020, Accepted 30 September 2020, Available online 6 November 2020.
Published: November 6, 2020
Cell Metabolism
Available online 6 November 2020 In Press
doi: doi.org/10.1016/j.cmet.2020.10.002
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pGADT7-Rec
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