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- LSM BIO
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- PVT11319
- 2026年03月16日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pFastBac1-Gus
- 库存：
10
- 供应商：
LSMBIO
- 规格：
质粒干粉
pFastBac1-Gus
Catalog No. PVT11319 Packing 2ug
pFastBac1-Gus Description
PFastBac1-Gus is an intracellular plasmid expressing insect cell baculovirus.
pFastBac1-Gus Information
Promoter: PH promoter
Replicon: pUC
Prokaryotic resistance: ampicillin Amp
Screening marker: gentamycin Gen
Clone strain: Escherichia coli DH5 alpha
Culture conditions: 37 C
pFastBac1-Gus Sequence
LOCUS Exported 6661 bp ds-DNA circular SYN 13-OCT-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..6661
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 2..457
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 484..588
/gene="bla"
/label=AmpR promoter
CDS 589..1449
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 1620..2208
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
mobile_element 2511..2735
/mobile_element_type="transposon:Tn7"
/note="mini-Tn7 element (right end of the Tn7 transposon)"
CDS complement(2802..3335)
/codon_start=1
/gene="aacC1"
/product="gentamycin acetyltransferase"
/label=GmR
/note="confers resistance to gentamycin"
/translation="MLRSSNDVTQQGSRPKTKLGMGIIRTCRLGPDQVKSMRAALD
LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
EEVMHFDIDPSTAT"
promoter complement(3524..3552)
/gene="intI1 (promoter lies within the coding sequence)"
/label=Pc promoter
/note="class 1 integron promoter"
promoter 3905..3996
/gene="polh from Autographa californica"
/label=polyhedrin promoter
/note="promoter for the baculovirus polyhedrin gene"
CDS 4081..5892
/codon_start=1
/gene="uidA (or gusA) from E. coli"
/product="beta-glucuronidase"
/label=GUS
/translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
polyA_signal 6152..6286
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
mobile_element 6315..6480
/mobile_element_type="transposon:Tn7"
/note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
421 aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat
481 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
541 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
601 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
661 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
721 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
781 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
841 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
901 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
961 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
1021 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
1081 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
1141 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
1201 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
1261 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
1321 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
1381 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
1441 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
1501 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
1561 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
1621 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
1681 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
1741 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
1801 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
1861 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
1921 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
1981 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg
2041 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
2101 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
2161 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
2221 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
2281 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
2341 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
2401 cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct
2461 ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga
2521 caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag
2581 acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt
2641 tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga
2701 ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac
2761 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg
2821 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg
2881 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca
2941 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact
3001 gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc
3061 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta
3121 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct
3181 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg
3241 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg
3301 ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca
3361 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa
3421 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
3481 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca
3541 ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
3601 cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc
3661 ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg
3721 cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt
3781 ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt
3841 gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc ggaatattaa
3901 taggatcatg gagataatta aaatgataac catctcgcaa ataaataagt attttactgt
3961 tttcgtaaca gttttgtaat aaaaaaacct ataaatattc cggattattc ataccgtccc
4021 accatcgggc gcggatccat atatagggcc cgggttataa ttacctcagg tcgacgtccc
4081 atggtccgtc ctgtagaaac cccaacccgt gaaatcaaaa aactcgacgg cctgtgggca
4141 ttcagtctgg atcgcgaaaa ctgtggaatt gatcagcgtt ggtgggaaag cgcgttacaa
4201 gaaagccggg caattgctgt gccaggcagt tttaacgatc agttcgccga tgcagatatt
4261 cgtaattatg cgggcaacgt ctggtatcag cgcgaagtct ttataccgaa aggttgggca
4321 ggccagcgta tcgtgctgcg tttcgatgcg gtcactcatt acggcaaagt gtgggtcaat
4381 aatcaggaag tgatggagca tcagggcggc tatacgccat ttgaagccga tgtcacgccg
4441 tatgttattg ccgggaaaag tgtacgtatc accgtttgtg tgaacaacga actgaactgg
4501 cagactatcc cgccgggaat ggtgattacc gacgaaaacg gcaagaaaaa gcagtcttac
4561 ttccatgatt tctttaacta tgccggaatc catcgcagcg taatgctcta caccacgccg
4621 aacacctggg tggacgatat caccgtggtg acgcatgtcg cgcaagactg taaccacgcg
4681 tctgttgact ggcaggtggt ggccaatggt gatgtcagcg ttgaactgcg tgatgcggat
4741 caacaggtgg ttgcaactgg acaaggcact agcgggactt tgcaagtggt gaatccgcac
4801 ctctggcaac cgggtgaagg ttatctctat gaactgtgcg tcacagccaa aagccagaca
4861 gagtgtgata tctacccgct tcgcgtcggc atccggtcag tggcagtgaa gggcgaacag
4921 ttcctgatta accacaaacc gttctacttt actggctttg gtcgtcatga agatgcggac
4981 ttacgtggca aaggattcga taacgtgctg atggtgcacg accacgcatt aatggactgg
5041 attggggcca actcctaccg tacctcgcat tacccttacg ctgaagagat gctcgactgg
5101 gcagatgaac atggcatcgt ggtgattgat gaaactgctg ctgtcggctt taacctctct
5161 ttaggcattg gtttcgaagc gggcaacaag ccgaaagaac tgtacagcga agaggcagtc
5221 aacggggaaa ctcagcaagc gcacttacag gcgattaaag agctgatagc gcgtgacaaa
5281 aaccacccaa gcgtggtgat gtggagtatt gccaacgaac cggatacccg tccgcaaggt
5341 gcacgggaat atttcgcgcc actggcggaa gcaacgcgta aactcgaccc gacgcgtccg
5401 atcacctgcg tcaatgtaat gttctgcgac gctcacaccg ataccatcag cgatctcttt
5461 gatgtgctgt gcctgaaccg ttattacgga tggtatgtcc aaagcggcga tttggaaacg
5521 gcagagaagg tactggaaaa agaacttctg gcctggcagg agaaactgca tcagccgatt
5581 atcatcaccg aatacggcgt ggatacgtta gccgggctgc actcaatgta caccgacatg
5641 tggagtgaag agtatcagtg tgcatggctg gatatgtatc accgcgtctt tgatcgcgtc
5701 agcgccgtcg tcggtgaaca ggtatggaat ttcgccgatt ttgcgacctc gcaaggcata
5761 ttgcgcgttg gcggtaacaa gaaagggatc ttcactcgcg accgcaaacc gaagtcggcg
5821 gcttttctgc tgcaaaaacg ctggactggc atgaacttcg gtgaaaaacc gcagcaggga
5881 ggcaaacaat gaatcaacaa ctctcctggc gcaccatcgt cggctacagc ctcggtgggg
5941 aattcaaagg cctacgtcga cgagctcact agtcgcggcc gctttcgaat ctagagcctg
6001 cagtctcgag gcatgcggta ccaagcttgt cgagaagtac tagaggatca taatcagcca
6061 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
6121 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
6181 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
6241 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatctgat cactgcttga
6301 gcctaggaga tccgaaccag ataagtgaaa tctagttcca aactattttg tcatttttaa
6361 ttttcgtatt agcttacgac gctacaccca gttcccatct attttgtcac tcttccctaa
6421 ataatcctta aaaactccat ttccacccct cccagttccc aactattttg tccgcccaca
6481 gcggggcatt tttcttcctg ttatgttttt aatcaaacat cctgccaact ccatgtgaca
6541 aaccgtcatc ttcggctact ttttctctgt cacagaatga aaatttttct gtcatctctt
6601 cgttattaat gtttgtaatt gactgaatat caacgcttat ttgcagcctg aatggcgaat
6661 g
//
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文献和实验Patterns in the localization of compounds, metabolic processes, and regulatory machineries at the cellular and subcellular levels are studied by scientific disciplines, called cytochemistry (biochemistry of the cell, cellular topochemistry
(Janine’s protocol) 1. Add 200µl of acetone, vortex briefly. 2. Remove acetone and pipette into flat-bottom 96-well plate (polystyrene) 3. Centrifuge plate at 3000 x g for 3-5 minutes. 4. Invert plate carefully and blot dry
一、实验试剂 GUS Buffer (500 ml) 2.0478 g Na2HPO4 1.2688 g NaH2PO4 (=50 mM NaPi pH7.0) 10 ml 0.5 M EDTA (=10 mM) 0.5 g Triton X-100 0.5 g N-Lauroylsarcosine Sodium Salt (=0.1%) Extraction Buffer (500ul
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