pSH47 Plasmid

pSH47

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pSH47,Plasmid pSH47,pSH47 vector

pSH47 plasmid information

Carrier name: pSH47

Plasmid type: yeast gene knockout carrier

High copy / low copy: high copy

Promoter: GAL

Cloning methods: polyclonal sites, restrictive endonucleases

Carrier size: 6979 BP

5'sequencing primers and sequences: M13-F:TGTAAAACGACGGCCAGT

3'sequencing primers and sequences: M13-R:CAGGAAACAGCTATGAC

Carrier screening markers: URA3

Carrier resistance: ampicin

Genbank No.: AF298782

Remarks: Cre enzyme yeast expression plasmid

Product directory number: -

Stability: stability

Composition type: inducible type

Virus / non virus: non virus

Use:Homologous recombination HR plasmid

pSH47 plasmid Sequence

  LOCUS       pSH47                6979 bp    DNA     circular SYN 11-MAR-2002  DEFINITION  Recombinase expression vector pSH47, complete sequence.  ACCESSION   AF298782  VERSION     AF298782.1  GI:11344869  KEYWORDS    .  SOURCE      recombinase expression vector pSH47    ORGANISM  recombinase expression vector pSH47              artificial sequences; vectors.  REFERENCE   1  (bases 1 to 6979)    AUTHORS   Guldener,U., Heck,S., Fielder,T., Beinhauer,J. and Hegemann,J.H.    TITLE     A new efficient gene disruption cassette for repeated use in              budding yeast    JOURNAL   Nucleic Acids Res. 24 (13), 2519-2524 (1996)    MEDLINE   96292254     PUBMED   8692690  REFERENCE   2  (bases 1 to 6979)    AUTHORS   Gueldener,U., Heinisch,J., Koehler,G.J., Voss,D. and Hegemann,J.H.    TITLE     A second set of loxP marker cassettes for Cre-mediated multiple              gene knockouts in budding yeast    JOURNAL   Nucleic Acids Res. 30 (6), E23 (2002)    MEDLINE   21882009     PUBMED   11884642  REFERENCE   3  (bases 1 to 6979)    AUTHORS   Gueldener,U., Hegemann,J.H., Heck,S., Fiedler,T. and Beinhauer,J.D.    TITLE     Direct Submission    JOURNAL   Submitted (23-AUG-2000) Institut fuer Mikrobiologie,              Heinrich-Heine-Universitaet, Universtitaetsstr. 1, Duesseldorf              40225, Germany  FEATURES             Location/Qualifiers       source          1..6979                       /organism="recombinase expression vector pSH47"                       /mol_type="other DNA"                       /db_xref="taxon:142964"                       /note="derived from Saccharomyces cerevisiae"       gene            479..1282                       /gene="URA3"       CDS             479..1282                       /gene="URA3"                       /codon_start=1                       /transl_table=11                       /product="orotidine-5'-phosphate decarboxylase"                       /protein_id="AAG34514.1"                       /db_xref="GI:11344870"       terminator      2066..2324                       /note="from CYC1"       gene            complement(2642..3673)                       /gene="Cre"       CDS             complement(2642..3673)                       /gene="Cre"                       /codon_start=1                       /transl_table=11                       /product="recombinase"                       /protein_id="AAG34515.1"                       /db_xref="GI:11344871"       promoter        complement(3782..4249)                       /note="from GAL1"       rep_origin      4692..4764       gene            complement(5467..6327)                       /gene="AmpR"       CDS             complement(5467..6327)                       /gene="AmpR"                       /codon_start=1                       /transl_table=11                       /product="ampicillin resistance protein"                       /protein_id="AAG34516.1"                       /db_xref="GI:11344872"  BASE COUNT     1939 a   1562 c   1608 g   1869 t      1 others  ORIGIN                1 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg         61 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt        121 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg        181 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt        241 gcaccatacc acagcttttc aattcaattc atcatttttt ttttattctt ttttttgatt        301 tcggtttctt tgaaattttt ttgattcggt aatctccgaa cagaaggaag aacgaaggaa        361 ggagcacaga cttagattgg tatatatacg catatgtagt gttgaagaaa catgaaattg        421 cccagtattc ttaacccaac tgcacagaac aaaaacctgc aggaaacgaa gataaatcat        481 gtcgaaagct acatataagg aacgtgctgc tactcatcct agtcctgttg ctgccaagct        541 atttaatatc atgcacgaaa agcaaacaaa cttgtgtgct tcattggatg ttcgtaccac        601 caaggaatta ctggagttag ttgaagcatt aggtcccaaa atttgtttac taaaaacaca        661 tgtggatatc ttgactgatt tttccatgga gggcacagtt aagccgctaa aggcattatc        721 cgccaagtac aattttttac tcttcgaaga cagaaaattt gctgacattg gtaatacagt        781 caaattgcag tactctgcgg gtgtatacag aatagcagaa tgggcagaca ttacgaatgc        841 acacggtgtg gtgggcccag gtattgttag cggtttgaag caggcggcag aagaagtaac        901 aaaggaacct agaggccttt tgatgttagc agaattgtca tgcaagggct ccctatctac        961 tggagaatat actaagggta ctgttgacat tgcgaagagc gacaaagatt ttgttatcgg       1021 ctttattgct caaagagaca tgggtggaag agatgaaggt tacgattggt tgattatgac       1081 acccggtgtg ggtttagatg acaagggaga cgcattgggt caacagtata gaaccgtgga       1141 tgatgtggtc tctacaggat ctgacattat tattgttgga agaggactat ttgcaaaggg       1201 aagggatgct aaggtagagg gtgaacgtta cagaaaagca ggctgggaag catatttgag       1261 aagatgcggc cagcaaaact aaaaaactgt attataagta aatgcatgta tactaaactc       1321 acaaattaga gcttcaattt aattatatca gttattaccc tatgcggtgt gaaataccgc       1381 acagatgcgt aaggagaaaa taccgcatca ggaaattgta aacgttaata ttttgttaaa       1441 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa       1501 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa       1561 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca       1621 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg       1681 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc       1741 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc       1801 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca       1861 gggcgcgtcg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg       1921 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg       1981 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta       2041 atacgactca ctatagggcg aattgggtac cggccgcaaa ttaaagcctt cgagcgtccc       2101 aaaaccttct caagcaaggt tttcagtata atgttacatg cgtacacgcg tctgtacaga       2161 aaaaaaagaa aaatttgaaa tataaataac gttcttaata ctaacataac tataaaaaaa       2221 taaataggga cctagacttc aggttgtcta actccttcct tttcggttag agcggatgtg       2281 gggggagggc gtgaatgtaa gcgtgacata actaattaca tgactcgagg tcgacaattc       2341 caaccttacc caagagttcg ccaaactcag acatcacttt angcaaaacc gcgccgtgct       2401 tcttcctcgg tggcattcat cacgaaatgt tcagcactac gcatactttt gacaggaaac       2461 gcaacggata ttgagtcaat atcaggcatt ctatcgctca gctttacagt gacaatgacg       2521 gctggcgact gaatattagt gcttacagac agcactacat attttccgtc gatgttgaaa       2581 tcctttctca tatgtcacca taaatatcaa ataattatag caatcattta cgcgttaatg       2641 gctaatcgcc atcttccagc aggcgcacca ttgcccctgt ttcactatcc agggtacgga       2701 tatagttcat gacaatattt acattggtcc agccaccagc ttgcatgatc tccggtattg       2761 aaactccagc gcgggccata tctcgcgcgg ctccgacacg ggcactgtgt ccagaccagg       2821 ccaggtatct ctgaccagag tcatccttag cgccgtaaat caatcgatga gttgcttcaa       2881 aaatcccttc cagggcgcga gttgatagct ggctggtggc agatggcgcg gcaacaccat       2941 tttttctgac ccggcaaaac aggtagttat tcggatcatc agctacacca gagacggaaa       3001 tccatcgctc gaccagttta gttaccccca ggctaagtgc cttctctaca cctgcggtgc       3061 taaccagcgt tttcgttctg ccaatatgga ttaacattct cccaccgtca gtacgtgaga       3121 tatctttaac cctgatcctg gcaatttcgg ctatacgtaa cagggtgtta taagcaatcc       3181 ccagaaatgc cagattacgt atatcctggc agcgatcgct attttccatg agtgaacgaa       3241 cctggtcgaa atcagtgcgt tcgaacgcta gagcctgttt tgcacgttca ccggcatcaa       3301 cgttttcttt tcggatccgc cgcataacca gtgaaacagc attgctgtca cttggtcgtg       3361 gcagcccgga ccgacgatga agcatgttta gctggcccaa atgttgctgg atagttttta       3421 ctgccagacc gcgcgcctga agatatagaa gataatcgcg aacatcttca ggttctgcgg       3481 gaaaccattt ccggttattc aacttgcacc atgccgccca cgaccggcaa acggacagaa       3541 gcattttcca ggtatgctca gaaaacgcct ggcgatccct gaacatgtcc atcaggttct       3601 tgcgaacctc atcactcgtt gcatcgaccg gtaatgcagg caaattttgg tgtacggtca       3661 gtaaattgga catttaacac tcagataatg gttttaagta aagtgtacag gatcggctct       3721 gcccctcgac ggtatcgata agcttgatat cgaattcctg cagcccgggg gatccactag       3781 ttctagaatc cggggttttt tctccttgac gttaaagtat agaggtatat taacaatttt       3841 ttgttgatac ttttattaca tttgaataag aagtaataca aaccgaaaat gttgaaagta       3901 ttagttaaag tggttatgca gtttttgcat ttatatatct gttaatagat caaaaatcat       3961 cgcttcgctg attaattacc ccagaaataa ggctaaaaaa ctaatcgcat tatcatccta       4021 tggttgttaa tttgattcgt tcatttgaag gtttgtgggg ccaggttact gccaattttt       4081 cctcttcata accataaaag ctagtattgt agaatcttta ttgttcggag cagtgcggcg       4141 cgaggcacat ctgcgtttca ggaacgcgac cggtgaagac gaggacgcac ggaggagagt       4201 cttccttcgg agggctgtca cccgctcggc ggcttctaat ccgtactaga gctccagctt       4261 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc       4321 tgtgtgaaat tgttatccgc tcacaattcc acacaacata ggagccggaa gcataaagtg       4381 taaagcctgg ggtgcctaat gagtgaggta actcacatta attgcgttgc gctcactgcc       4441 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg       4501 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc       4561 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac       4621 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa       4681 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca       4741 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc       4801 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata       4861 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta       4921 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca       4981 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga       5041 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg       5101 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg       5161 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg       5221 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag       5281 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa       5341 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat       5401 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc       5461 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc       5521 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc       5581 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc       5641 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc       5701 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt       5761 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc       5821 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa       5881 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt       5941 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg       6001 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc       6061 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa       6121 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt       6181 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt       6241 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag       6301 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta       6361 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat       6421 aggggttccg cgcacatttc cccgaaaagt gccacctggg tccttttcat cacgtgctat       6481 aaaaataatt ataatttaaa ttttttaata taaatatata aattaaaaat agaaagtaaa       6541 aaaagaaatt aaagaaaaaa tagtttttgt tttccgaaga tgtaaaagac tctaggggga       6601 tcgccaacaa atactacctt ttatcttgct cttcctgctc tcaggtatta atgccgaatt       6661 gtttcatctt gtctgtgtag aagaccacac acgaaaatcc tgtgatttta cattttactt       6721 atcgttaatc gaatgtatat ctatttaatc tgcttttctt gtctaataaa tatatatgta       6781 aagtacgctt tttgttgaaa ttttttaaac ctttgtttat ttttttttct tcattccgta       6841 actcttctac cttctttatt tactttctaa aatccaaata caaaacataa aaataaataa       6901 acacagagta aattcccaaa ttattccatc attaaaagat acgaggcgcg tgtaagttac       6961 aggcaagcga tccgtccta  //