pTrc-CKS
Search name
pTrc-CKS,Plasmid pTrc-CKS,pTrc-CKS vector
pTrc-CKS Informaiton
Promoter: Trc/lac
Replicon: ColE1 ori
Terminator: rrnB T1 Terminator
Plasmid classification: Escherichia coli vector; PET series expression plasmid
Plasmid size: 5.2kb
Plasmid label: N-CKS, C-6 x His, N-P3C
Prokaryotic resistance: ampicillin Amp
Cloned strain: DH5 alpha
Culture conditions: 37 centigrade, aerobic, LB
Expression host: BL21 (DE3)
Culture conditions: 37 centigrade, aerobic, LB
Inducement: IPTG or lactose and its analogues
5'sequencing primers: 5 ATC CAT GTT GCT GTT GCT CAG G 3
3'sequencing primers: 5 GAT TTA ATC TGT ATC AGG 3
pTrc-CKS Description
pTrc-CKS expression vector uses E.coliTrc promoter and protein engineering to transform CKS (CTP:CMP-3-deoxy-D-manno-octulosonate cytidylyltransferase or CMP-KDO synthetase) gene fusion expression, and selects the exclusive cleavage site of the 14 subtype 3C protease of human rhinovirus. CKS fusion expression is the patent of Abbot company of the United States. CKS fusion protein has successfully developed HIV and HCV recombinant antigen and obtained FDA certification in the United States. CKS is a highly stable and highly soluble ideal fusion partner. In addition to removing 8 amino acids from the CKS C terminal, the invention also changes the C end Met to Thr.
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