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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pUC57-Kanamycin
- 库存：
50
- 供应商：
LSMBIO
- 规格：
2ug
pUC57-Kanamycin
Search name
pUC57-Kanamycin,Plasmid pUC57-Kanamycin,pUC57-Kanamycin vector
pUC57-Kanamycin Information
Promoter: Lac/lac
Replicon: ColE1 ori
Plasmid classification: Escherichia coli vector; cloned plasmid
Plasmid size: 2579bp
Plasmid label: LacZ
Prokaryotic resistance: kanamycin Kan
Cloned strain: DH5 alpha
Culture conditions: 37 centigrade, aerobic, LB
5'sequencing primers: M13R:CAGGAAACAGCTATGACC
3'sequencing primers: M13F:TGTAAAACGACGGCCAGT
Note: selection of blue leukoplakia
pUC57-Kanamycin Description
pUC57-Kanamycin Sequence
LOCUS Exported File 2579 bp ds-DNA circular SYN 02-2-2015
DEFINITION .
ACCESSION .
VERSION .
KEYWORDS pUC57-Kan
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2579)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported 2015-2-2
FEATURES Location/Qualifiers
source 1..2579
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 379..395
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(489..505)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 513..529
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(537..567)
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 582..603
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(891..1479)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1657..2466)
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/note="KanR"
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
421 tgcatctaga tatcggatcc cgggcccgtc gactgcagag gcctgcatgc aagcttggcg
481 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
541 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
601 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
661 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
721 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
781 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
841 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
901 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
961 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
1021 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
1081 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
1141 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
1201 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
1261 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
1321 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
1381 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
1441 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
1501 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
1561 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaagccca
1621 atctgaataa tgttacaacc aattaaccaa ttctgattag aaaaactcat cgagcatcaa
1681 atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt
1741 ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg
1801 gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat
1861 aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag
1921 tttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc
1981 actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagac gaaatacgcg
2041 atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc
2101 cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt
2161 ttttccgggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt
2221 gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac
2281 atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc
2341 atacaagcga tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc
2401 atataaatca gcatccatgt tggaatttaa tcgcggcctc gacgtttccc gttgaatatg
2461 gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgatga
2521 tatattttta tcttgtgcaa tgtaacatca gagattttga gacacgggcc agagctgca
//
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文献和实验This protocol is for Mini (up to 20 µg) preparations of high-copy plasmid DNA from cultures of E. coli . Important notes before starting New users are strongly recommended to read Appendix A (page 21) at the end
Plasmid Profile Typing and Plasmid Fingerprinting
Plasmids are extra-chromosomal molecules of deoxyribonucleic acid (DNA) capable of autonomous replication. Such molecules have been identified in many bacterial genera and usually exist as covalently closed circular (CCC) molecules. Plasmids
质粒 plasmid 在细胞内整个世代稳定地传递给后代，但并不在染色体上而进行自主增殖的一类遗传因子的总称。该类因子的存在与否，对细胞的生存无关紧要。这个名词主要指细菌细胞内的因子，它们包括可在细胞间发生传递的F因子、 R因子、大肠杆菌素因子等。此外，还有各种本身不能传递的因子。在具有质粒细胞的后代中，有时会出现低频率的失去质粒的细菌。在质粒中，对那些能变成插入奇主染色体状态的特殊质粒，特称为附加体（episome）。
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