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- 2025年09月19日
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- 详细信息
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- 文献和实验
- 技术资料
- 保存条件：
负80摄氏度
- 保质期：
3个月
- 英文名：
AGL1 Agrobacterium Strain
- 库存：
50
- 供应商：
LSM BIO
- 规格：
100ul
AGL1 Agrobacterium Strain
STR3006 Packing 100ul
Store at -80鈩?/p>
AGL1 Agrobacterium Strain Genotype
C58 RecA (rif R/carbR) Ti pTiBo542DT-DNA (strepR) Succinamopine
AGL1 Agrobacterium Strain Information
Type Agrobacterium tumefaciens
Resistance: Rif (20ug/ml)/ Car(50 μg/ml)
Culture medium: YEB
Strain category: Agrobacterium tumefaciens
Culture conditions: 28 centigrade, aerobic, YEB
Transformation of plasmids: electric conversion and transformation
Preservation method: 30% glycerin, -80鈩?br /> Basic application: transgene
AGL1 Agrobacterium Strain Description
AGL1 Agrobacterium Strain was C58, RecA background, containing screening label, rifampicin resistant gene RIF and carbenicillin resistance gene carb in nuclear genes, in order to facilitate the transformation of the operation, the strains carrying Ti plasmid pTiBo542DT-DNA amber base without its transport function, the plasmid containing vir gene (VIR T-DNA gene was inserted into the plant genome required the T-DNA element, pTiBo542DT-DNA plasmid transfer function is damaged, but can help the binary vector T-DNA to successfully transfer). The pTiBo542DT-DNA Ti plasmid contains a screening tag: strep, giving AGL1 streptomycin resistance, which is suitable for transgenic operation of plants such as rice, Arabidopsis and poplar. AGL1 strain was cultured on LB or YEB at 28 centigrade aerobic culture, and 30% glycerol could be used to preserve the bacteria.
Caution:
1. This product is FOR RESEARCH USE ONLY!
2.Please kindly culture it as soon as possible then harvest and store at -80鈩?
3. Shipping condition 4鈩?(glycerol).
AGL1 Agrobacterium Strain Reference
Publication for STR3006 AGL1 Agrobacterium Strain
Article: CRISPR-Cas Methods
M. Tofazzal IslamPankaj K. BhowmikKutubuddin A. Molla
URI: doi.org/10.1007/978-1-0716-0616-2
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文献和实验Agrobacterium growth and transformation
. This is not very efficient (~200 colonies per microgram of DNA), but it is really easy.1. Pick a single colony of the Agrobacterium strain of choice and inoculate 3 ml of LB (or 2YT) in a 15 ml snap-cap tube (Falcon tube). Grow @ 30℃ overnight on a roller drum. Be sure
Growth and Storage of Agrobacterium
Proper preservation of strains is of great importance to all microbiologists. Working with contaminated or genetically changed cultures, or facing the loss of a strain are annoyances that often may be easily prevented. A reliable preservation
Electroporation of Agrobacterium tumefaciens
Agrobacterium tumefaciens is a soil bacterium that causes tumors on dicotyledonous plants. Virulent strains harbor a large plasmid, the Ti (tumor-inducing) plasmid, which is involved in tumorigenesis. A small segment of this plasmid, the T
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