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- LSM Bio
- CHC00052
- 2025年07月16日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
负80摄氏度
- 保质期：
6month
- 英文名：
MC1061 chemically E.coli Express Competent Cells
- 库存：
货源充足
- 供应商：
LSM Bio
- 规格：
0.1ml*10
MC1061 chemically Competent Cells
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文献和实验a chilled, sterile pipette tip to transfer the competent cells to chilled, sterile 17 x 100-mm polypropylene tubes. Store the cells on ice. Glass tubes should not be used since they lower the efficiency of transformation by ~10-fold
Transformation DNA fragments (or plasmid DNA) into competent E. coli
Transformation DNA fragments (or plasmid DNA) into competent E. coli * Caution: Use aerosol protecting tips if selection of transformed cells is not based on X-gal strategy. 1.Remove competent cells (E.coli DH5aTM from GIBCO BRL) from -70 ℃
,etc.).If a cooling shaker is not available- grow the cells at room temperature.More discussions on making competent cell as well as references can be found in TIBS articles "Preparing μltra-competent E.coli" and "Better competent cells"It is also possible
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