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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负80摄氏度
- 保质期：
6month
- 英文名：
GV3101 Electro Agrobacterium Express Competent Cells
- 库存：
货源充足
- 供应商：
LSM Bio
- 规格：
0.5ml*10
GV3101 Electro Competent Cells
Packing
GV3101 Electro Competent Cells锛? 50μl/tube
pCAMBIA2301锛坈ontrol vector锛?0ng/μl ) 10μl
Storage -80鈩冿紙12month锛?/p>
GV3101 Electro Competent Cells Genotype
C58 (rif r) Ti pMP90 (pTiC58DT-DNA) ( gentr) Nopaline
Plasmid transformation efficiency
pCAMBIA2301 (size:11633 bp) >105 cfu/μgDNA锛?/p>
pCAMBIA2301-ZH锛坰ize:40 kd锛?×103 cfu/μg DNA
Caution
1.STORAGE AND HANDLING: Competent cells should be stored at–80°C. Storage at –20°C will result in a significant decrease in transformation efficiency. Cells lose efficiency whenever they are warmed above –80°C, even if they do not thaw.
2. This product is FOR RESEARCH USE ONLY!
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文献和实验Agrobacterium growth and transformation
Growth and storage of Agrobacterium tumefaciensStrain GV3101: resistant to gentamycin and rifampicin so add 25-50 ug/ml Gentamycin, 10 ug/ ml rifampicin on plates or in liquid media for selection. GV3101 is sensitive to kanamycin (or chloramphenicol
Agrobacterium growth and transformation
more cells. Therefore, electroporation using frozen competent cells is 102-103 more efficient than the freeze/thaw method. According to the strain of Agrobacterium used, different antibiotics are required. The most commonly used strains LBA4404 and GV3101
Agrobacterium growth and transformation (农杆菌培养及转化)
Growth and storage of Agrobacterium tumefaciens(农杆菌培养及转化) Strain GV3101: resistant to gentamycin and rifampicin so add 25-50 ug/ml Gentamycin, 10 ug/ ml rifampicin on plates or in liquid media for selection. GV3101
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