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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
负80摄氏度
- 保质期:
6month
- 英文名:
MSU440 Electro Agrobacterium Express Competent Cells
- 库存:
货源充足
- 供应商:
LSM Bio
- 规格:
0.5ml*10
MSU440 Electro Competent Cells
Packing
MSU440 Electro Competent Cells锛? 50μl/tube
pCAMBIA2301锛坈ontrol vector锛?0ng/μl ) 10μl
Storage -80鈩冿紙12month锛?/span>
MSU440 Electro Competent Cells Genotype
Agrobacterium rhizogenes(strR ) MSU440 Ri (agropine type)
Plasmid transformation efficiency
pCAMBIA2301 (size:11633 bp) >105 cfu/μgDNA锛?/span>
pCAMBIA2301-ZH锛坰ize:40 kd锛?×103 cfu/μg DNA
Caution
1.STORAGE AND HANDLING: Competent cells should be stored at–80°C. Storage at –20°C will result in a significant decrease in transformation efficiency. Cells lose efficiency whenever they are warmed above –80°C, even if they do not thaw.
2. This product is FOR RESEARCH USE ONLY!
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文献和实验a chilled, sterile pipette tip to transfer the competent cells to chilled, sterile 17 x 100-mm polypropylene tubes. Store the cells on ice. Glass tubes should not be used since they lower the efficiency of transformation by ~10-fold
5α时需要注意什么?·······································2 Q-8:使用E.coli Competent Cells HB101时需要注意什么?·················································2 Q-9:使用电转化感受态细胞时需要注意什么?····································································
(Competent cells):受体细胞经过一些特殊方法(如:CaCl,RuCl等化学试剂法)的处理后,细胞膜的通透性发生变化,成为能容许多有外源DNA的载体分子通过的感受态细胞(competent cell) 。 转化(transformation):是将异源DNA分子引入一细胞株系,使受体细胞获得新的遗传性状的一种手段,是基因工程等研究领域的基本实验技术。 电转化法:使用低盐缓冲液或水洗制备的感受态细胞,通过高压脉冲的作用将载体DNA分子导入受体细胞。 克隆的筛选:主要用不同抗生
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