Protein A| Human Protein A & S

蛋白分离纯化概述（An overview of protein isolation）

Isolating a protein may be compared to playing a game of golf. In golf, the player is faced with a series of problems, each unique and yet similar to problems previously encountered. In facing each problem the player must analyse

【原创】Protein A Sepharose 之进化篇。。。

[img][/img]Protein A 柱之进化 Protein A是一种金黄色葡萄球菌细胞壁蛋白质，能特异性地与人和哺乳动物抗体（主要是IgG）的Fc区结合。因而，将Protein A 与琼脂糖凝胶以一定的方式结合，可制备用于抗体纯化的亲和填料。 早期的Protein A柱结合的都是天然Protein A。天然Protein A由5个IgG结合域和其它未知功能的非Fc结合域组成，分子量约42KD，结构如图1所示。这种柱子对IgG的亲和能力很强，可以吸附大量的lgG。但同时，天然

蛋白质纯化的原理和方法（Protein Purification Principles and Methods）

and Hydrophobic-Interactions •“Weak” interactions are important for structure and function (activity) of the protein ÆIn most cases the purification must be gentle! Before the purification… •Cultivation of bacteria •Cell disruption