Human SDCBP ELISA Kit
Product Name:Human SDCBP ELISA Kit
Packing:96T
Catalog No.:ELA-E14986h
Gene Name:SDCBP
Detect Range:0.312-20 ng/mL
Sensitivity:0.12ng/ml
Target Protein Name:Human Syntenin-1
Alternative Name:Syntenin-1, Melanoma differentiation-associated protein 9, MDA-9, Pro-TGF-alpha cytoplasmic domain-interacting protein 18, TACIP18, Scaffold protein Pbp1, Syndecan-binding protein 1,
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human SDCBP ELISA Kit allows for the in vitro quantitative determination of Human Syntenin-1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human SDCBP ELISA Kit has been pre-coated with an SDCBP antibody specific to Human Syntenin-1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Syntenin-1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Syntenin-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Syntenin-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!