- ￥2890
- LSM Bio
- 0.31-20NG/ML
- 0.1NG/ML
- 国产(香港)
- EF009556
- 2026年05月21日
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- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSMBIO
- 检测范围：
0.31-20NG/ML
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human FANCD2-phospho S330
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.1NG/ML
- 规格：
96T
FANCD2-phospho S330 ELISA KIT|Human
Catalog EF009556
Packing 48T/96T
Reactivity Human
Detect Range 0.313-20ng/ml
FANCD2-phospho S330 ELISA KIT Sensitivity< 0.188ng/ml
Application FANCD2-phospho S330 ELISA KIT|Human is for detecting the concentration of Human FANCD2-phospho S330 in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of FANCD2-phospho S330 ELISA KIT|Human
The microtiter plate provided in FANCD2-phospho S330 ELISA KIT|Human has been pre-coated with an Human FANCD2-phospho S330 antibody specific to Human FANCD2-phospho S330. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human FANCD2-phospho S330antibody preparation specific for Human FANCD2-phospho S330 then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human FANCD2-phospho S330, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human FANCD2-phospho S330in the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that FANCD2-phospho S330 ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
in many signaling pathways typically associated with human malignancies. Phospho?motif antibodies are engineered to recognize proteins that contain a phosphorylated residue in the context of a specific motif. They are raised against a library of phospho?peptides
调至 1μg/μl。 4. ELISA 检测：按试剂盒说明书完成板平衡、加样孵育、洗板、酶标抗体孵育、显色终止与读数，结合标准曲线得出 IFN-γ 浓度。 四、操作要点速记 1.组织样本新鲜处理，全程低温操作，裂解液现配现用； 2. 蛋白定量时标准品与样本同步孵育，稀释采用倍比稀释减少误差； 3. ELISA 实验需每次重绘标准曲线，洗板充分、严格把控显色与终止时间。 免疫检测试剂盒： 货号-规格 名称 abs510001-96T Human IL-2 ELISA Kit (Faster
就可进行一次检测。3、更高的灵敏度。分析灵敏度高达2.8pg/mL。4、更高的灵敏度、更宽的检测范围和更好的重复性。CBA技术使蛋白定量的分析灵敏度高达pg/ml级别,检测范围达0-5000pg/ml，所有分析只需一组标准曲线。根据抗体的荧光强度对目的蛋白定量，排除了ELISA反应中酶联放大产生的假阳性。5、检测对象的灵活组合—BD CBA Flex Set。CBA kit是同时检测试剂盒配好的6个指标，形式比较固定。为满足不同客户的实际需要和研究兴趣，BD于2005年初推出了CBA Flexset
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