- ￥2890
- LSM Bio
- 0.31-20NG/ML
- 0.1NG/ML
- 国产
- EF009556
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.31-20NG/ML
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human FANCD2-phospho S330
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.1NG/ML
- 规格：
96T
FANCD2-phospho S330 ELISA KIT|Human
Catalog EF009556
Packing 48T/96T
Reactivity Human
Detect Range 0.313-20ng/ml
FANCD2-phospho S330 ELISA KIT Sensitivity< 0.188ng/ml
Application FANCD2-phospho S330 ELISA KIT|Human is for detecting the concentration of Human FANCD2-phospho S330 in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of FANCD2-phospho S330 ELISA KIT|Human
The microtiter plate provided in FANCD2-phospho S330 ELISA KIT|Human has been pre-coated with an Human FANCD2-phospho S330 antibody specific to Human FANCD2-phospho S330. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human FANCD2-phospho S330antibody preparation specific for Human FANCD2-phospho S330 then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human FANCD2-phospho S330, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human FANCD2-phospho S330in the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that FANCD2-phospho S330 ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
in many signaling pathways typically associated with human malignancies. Phospho?motif antibodies are engineered to recognize proteins that contain a phosphorylated residue in the context of a specific motif. They are raised against a library of phospho?peptides
就可进行一次检测。3、更高的灵敏度。分析灵敏度高达2.8pg/mL。4、更高的灵敏度、更宽的检测范围和更好的重复性。CBA技术使蛋白定量的分析灵敏度高达pg/ml级别,检测范围达0-5000pg/ml，所有分析只需一组标准曲线。根据抗体的荧光强度对目的蛋白定量，排除了ELISA反应中酶联放大产生的假阳性。5、检测对象的灵活组合—BD CBA Flex Set。CBA kit是同时检测试剂盒配好的6个指标，形式比较固定。为满足不同客户的实际需要和研究兴趣，BD于2005年初推出了CBA Flexset
有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂，增加了操作步骤，在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法：1， TORCH及传染病试剂盒（间接法），见2.2.32， TORCH-IgM捕获法特色：包被抗体，标记抗原原理：3， 细胞因子试剂盒采用的方法路线（ABC-ELISA）原理产品特色：采用ABC法，灵敏度更高，特异性更强。生物素抗体和酶联物是浓缩的，使用前需用相应的缓冲液稀释。酶联物可以通用
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