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- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSMBIO
- 检测范围:
0.31-20NG/ML
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Caspase 2
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.1NG/ML
- 规格:
96T
Caspase 2 ELISA KIT|Human
Catalog EF008400
Packing 48T/96T
Reactivity Human
Detect Range 0.156-10ng/ml
Caspase 2 ELISA KIT Sensitivity< 0.094ng/ml
Application Caspase 2 ELISA KIT|Human is for detecting the concentration of Human Caspase 2 in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of Caspase 2 ELISA KIT|Human
The microtiter plate provided in Caspase 2 ELISA KIT|Human has been pre-coated with an Human Caspase 2 antibody specific to Human Caspase 2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human Caspase 2antibody preparation specific for Human Caspase 2 then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Caspase 2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human Caspase 2in the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that Caspase 2 ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!
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文献和实验Cell Death Detection NMP ELISA kit (QIA20) 或Nucleosome ELISA kit 的实验结果相互印证。 14、高通量Caspase-3 Activity Assay Kit 目录号:HTS02 包装:100T/Kit 500T/Kit 1000T/Kit 本试剂盒提供了一种可用于各种来源的Caspase-3 的体外快速检测手段(细胞裂解液)。试剂盒中提供了以下试剂:荧光标记的Caspase-3 底物,Caspase
调至 1μg/μl。 4. ELISA 检测:按试剂盒说明书完成板平衡、加样孵育、洗板、酶标抗体孵育、显色终止与读数,结合标准曲线得出 IFN-γ 浓度。 四、操作要点速记 1.组织样本新鲜处理,全程低温操作,裂解液现配现用; 2. 蛋白定量时标准品与样本同步孵育,稀释采用倍比稀释减少误差; 3. ELISA 实验需每次重绘标准曲线,洗板充分、严格把控显色与终止时间。 免疫检测试剂盒: 货号-规格 名称 abs510001-96T Human IL-2 ELISA Kit (Faster
性脊柱炎相关指标产品推荐: 靶标 抗体 重组蛋白 试剂盒 HLA-B27 HLA-B27 antibody HLA-B27 recombinant protein HLA-B27 ELISA Kit Caspase-1 Caspase-1 antibody Caspase-1 recombinant protein Caspase-1 ELISA Kit
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