- ￥2890
- LSM Bio
- 0.15-10NG/ML
- 0.078NG/ML
- 进口
- EF007357
- 2025年08月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10NG/ML
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human NMDA Antibody IgG
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078NG/ML
- 规格：
96T
NMDA Antibody IgG ELISA KIT|Human
Catalog EF007357
Packing 48T/96T
Reactivity Human
Detect Range 1.563-100ng/ml
NMDA Antibody IgG ELISA KIT Sensitivity< 0.938ng/ml
Application NMDA Antibody IgG ELISA KIT|Human is for detecting the concentration of Human NMDA Antibody IgG in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of NMDA Antibody IgG ELISA KIT|Human
The microtiter plate provided in NMDA Antibody IgG ELISA KIT|Human has been pre-coated with an Human NMDA Antibody IgG antibody specific to Human NMDA Antibody IgG. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human NMDA Antibody IgGantibody preparation specific for Human NMDA Antibody IgG then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human NMDA Antibody IgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human NMDA Antibody IgGin the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that NMDA Antibody IgG ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!
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文献和实验consists of a cooperation between monocytes and cytophilic antibodies able to bind to the Fc receptors present on the monocyte surface. Thus human IgG1 and IgG3 are the main isotypes effective in ADCI, whereas IgG2, IgG4, and IgM are ineffective
Quantification of Human IgG and Related Fc Fusion Proteins by a Human IgG/Fc Capture ELISA
of recombinant human IgGs after production and purification for further assays. Here, we describe a human IgG/Fc capture enzyme linked immunosorbent assay (ELISA) which is simple, robust, inexpensive and specific for all types of human IgGs and related Fc fusion
1 to a tube. 2) Add 20 μl of Antibody Mix. 3) Mix well and incubate for 20 min at 2–8°C. 4) Wash the cells by adding 2 ml Buffer 1. Mix well by tilting the tube several times and centrifuge at 300 x g for 10 min at 2–
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