- ￥2890
- LSM Bio
- 0.15-10NG/ML
- 0.078NG/ML
- 进口
- EF006793
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10NG/ML
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Indoxyl Sulphate
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078NG/ML
- 规格：
96T
Indoxyl Sulphate ELISA KIT|Human
Catalog EF006793
Packing 48T/96T
Reactivity Human
Detect Range 0.156-10ng/ml
Indoxyl Sulphate ELISA KIT Sensitivity< 0.094ng/ml
Application Indoxyl Sulphate ELISA KIT|Human is for detecting the concentration of Human Indoxyl Sulphate in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of Indoxyl Sulphate ELISA KIT|Human
The microtiter plate provided in Indoxyl Sulphate ELISA KIT|Human has been pre-coated with an Human Indoxyl Sulphate antibody specific to Human Indoxyl Sulphate. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human Indoxyl Sulphateantibody preparation specific for Human Indoxyl Sulphate then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Indoxyl Sulphate, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human Indoxyl Sulphatein the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that Indoxyl Sulphate ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
salt NBT, (Altman, 1972) can be used in conjunction with X-Phos as the final electron acceptor for the indoxyl dimerization reaction. When reduced, NBT forms a purple precipitate. Of the cloned phosphatases, the human placental alkaline
有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂，增加了操作步骤，在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法：1， TORCH及传染病试剂盒（间接法），见2.2.32， TORCH-IgM捕获法特色：包被抗体，标记抗原原理：3， 细胞因子试剂盒采用的方法路线（ABC-ELISA）原理产品特色：采用ABC法，灵敏度更高，特异性更强。生物素抗体和酶联物是浓缩的，使用前需用相应的缓冲液稀释。酶联物可以通用
的原理用于分子诊断方面：TrimGen公司的KRAS Mutation Detection Kit在96孔板里一次能实现7/8个KRAS突变位点的检测。 另外基于相同原理的技术还有PPT-ELISA。这是用来筛截短突变的，相关文章见：1，Rapid screen for truncating ATM mutations by PTT-ELISA . 2，An ELISA-based high throughput protein truncation test
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