AP ELISA KIT|Human

AP ELISA KIT|Human

Catalog  EF006606

Packing 48T/96T

Reactivity Human

Detect Range 1.563-100ng/ml

AP ELISA KIT Sensitivity< 0.938ng/ml

Application AP ELISA KIT|Human  is for detecting the concentration of Human AP in serum, plasma, tissue homogenates and other biological fluids.

Storage condition 6 month at 4 Centigrade

 

Principle of AP ELISA KIT|Human

The microtiter plate provided in AP ELISA KIT|Human has been pre-coated with an Human AP antibody specific to Human AP . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human AP antibody preparation specific for Human AP then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human AP , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human AP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

 

*Please noted that AP ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!