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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSMBIO
- 检测范围:
0.15-10NG/ML
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human ARHGAP35
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078NG/ML
- 规格:
96T
ARHGAP35 ELISA KIT|Human
Catalog EF005043
Packing 48T/96T
Reactivity Human
Detect Range 0.156-10ng/ml
ARHGAP35 ELISA KIT Sensitivity< 0.094ng/ml
Application ARHGAP35 ELISA KIT|Human is for detecting the concentration of Human ARHGAP35 in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of ARHGAP35 ELISA KIT|Human
The microtiter plate provided in ARHGAP35 ELISA KIT|Human has been pre-coated with an Human ARHGAP35 antibody specific to Human ARHGAP35. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human ARHGAP35antibody preparation specific for Human ARHGAP35 then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human ARHGAP35, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human ARHGAP35in the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that ARHGAP35 ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!
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文献和实验小鼠白细胞介素-35(IL-35)酶联免疫分析(ELISA)
小鼠 白细胞介素 - 35( IL- 35) 酶联免疫 分析( ELISA ) 试剂 盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于测定小鼠血清,血浆及相关液体样本中 白细胞介素35(IL-35)的 含量。 实验原理 : 本试剂盒应用双抗体夹心法测定 标本 中 小鼠白细胞介素35 (IL- 35 ) 水平。用纯化的 小鼠白细胞介素35 抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入 白细胞介素35
人 白细胞介素 - 35( IL- 35) 酶联免疫 分析( ELISA ) 试剂 盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于测定人血清,血浆及相关液体样本中 白细胞介素35(IL-35)的 含量。 实验原理 : 本试剂盒应用双抗体夹心法测定 标本 中 人白细胞介素35 (IL- 35 ) 水平。用纯化的 人白细胞介素35 抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入 白细胞介素35
volume of 35 ml at room temperature. 2) Add the diluted buffy coat on top of 15 ml of Lymphoprep. 3) Centrifuge at 160 x g for 20 minutes at 20°C. Allow to decellerate without brakes. 4) Remove 20 ml of supernatant
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