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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10NG/ML
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Rho GTPase-activating protein 35,ARHGAP35
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078NG/ML
- 规格:
96T
Human Rho GTPase- activating protein 35, ARHGAP35 ELISA KIT
Product Name:Human Rho GTPase- activating protein 35, ARHGAP35 ELISA KIT
Packing:96T
Catalog No.:ELI-52597h
Gene Name:ARHGAP35
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Rho GTPase-activating protein 35(ARHGAP35
Alternative Name:ARHGAP35,Rho GTPase-activating protein 35(ARHGAP35
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Rho GTPase- activating protein 35, ARHGAP35 ELISA KIT allows for the in vitro quantitative determination of Rho GTPase-activating protein 35(ARHGAP35 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Rho GTPase- activating protein 35, ARHGAP35 ELISA KIT has been pre-coated with an Rho GTPase-activating protein 35(ARHGAP35 antibody specific to Rho GTPase-activating protein 35(ARHGAP35 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Rho GTPase-activating protein 35(ARHGAP35 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Rho GTPase-activating protein 35(ARHGAP35, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Rho GTPase-activating protein 35(ARHGAP35 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Biosensors for Characterizing the Dynamics of Rho Family GTPases in Living Cells
., and Danuser, G. 2009. Coordination of Rho GTPase activities during cell protrusion. Nature 461:99‐103. Michaelson, D., Silletti, J., Murphy, G., D'Eustachio, P., Rush, M
Overview of Protein Microarrays
. Hu, S., Xie, Z., Onishi, A., Yu, X., Jiang, L., Lin, J., Rho, H.‐s., Woodard, C., Wang, H., and Jeong, J.‐S. 2009. Profiling the human protein‐DNA interactome reveals ERK2 as a transcriptional repressor
Introduction of human 14-3-3 proteins
The human and bovine 14-3-3 eta protein mRNAs are highly conservedEvolutionary conservation of the 14-3-3 proteinExpression and structural analysis of 14-3-3 proteinsCrystal structure of the zeta isoform of the 14-3-3 proteinStructure of a 14
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