- ￥2890
- LSM Bio
- 0.15-10NG/ML
- 0.078NG/ML
- 国产(香港)
- EF001761
- 2026年04月29日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSMBIO
- 检测范围：
0.15-10NG/ML
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Hunan PI3K p87
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078NG/ML
- 规格：
96T
PI3K p87 ELISA KIT|Human
Catalog EF001761
Packing 48T/96T
Reactivity Human
Detect Range 0.313-20ng/ml
PI3K p87 ELISA KIT Sensitivity< 0.188ng/ml
Application PI3K p87 ELISA KIT|Human is for detecting the concentration of Human PI3K p87 in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of PI3K p87 ELISA KIT|Human
The microtiter plate provided in PI3K p87 ELISA KIT|Human has been pre-coated with an Human PI3K p87 antibody specific to Human PI3K p87. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human PI3K p87antibody preparation specific for Human PI3K p87 then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human PI3K p87, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human PI3K p87in the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that PI3K p87 ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!
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文献和实验yys700714 请问如何用免疫沉淀法测PI3K活性呢？我查到相关文献，写得很清楚的，不过还是不太懂，不知需要准备什么试剂，需要什么仪器测定，TLC是什么啊，是不是需要荧光图像仪？很多疑问，请求做过的高手给予指点。多谢！！ yhwangcams 建议可以使用Echelon的PI3-K ELISA试剂盒。 http://www.echelon-inc.com/ccp1231-pi3-k-elisa-kit-k
cells/mL) were incubated with LPS (2.5 μg/mL) in the presence or absence of hydroquinone for 6 hours. Supernatants were assayed for the levels of TNF-α, IL-1β, and IL-6 using a mouse TNF-α ELISA kit (Amersham, Little Chalfont, Buckinghamshire, UK).2
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