- ￥2890
- LSM Bio
- 0.15-10NG/ML
- 0.078NG/ML
- 国产
- EF001337
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10NG/ML
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Hunan alpha 1 microglobulin
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078NG/ML
- 规格：
96T
alpha 1 microglobulin ELISA KIT|Human
Catalog EF001337
Packing 48T/96T
Reactivity Human
Detect Range 0.625-40ng/ml
alpha 1 microglobulin ELISA KIT Sensitivity< 0.375ng/ml
Application alpha 1 microglobulin ELISA KIT|Human is for detecting the concentration of Human alpha 1 microglobulin in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of alpha 1 microglobulin ELISA KIT|Human
The microtiter plate provided in alpha 1 microglobulin ELISA KIT|Human has been pre-coated with an Human alpha 1 microglobulin antibody specific to Human alpha 1 microglobulin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human alpha 1 microglobulinantibody preparation specific for Human alpha 1 microglobulin then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human alpha 1 microglobulin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human alpha 1 microglobulinin the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that alpha 1 microglobulin ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!
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酶的活性。Intergen公司的TRAP-eze Telemerase Detection Kit在1996年率先推出。它提供特定的寡核苷酸底物，分别与底物及端粒重复序列配对的引物。如果待测样本中含有端粒酶活性，就能在底物上接上不同个数的6碱基(GGTTAG)端粒重复序列，通过PCR反应，产物电泳检测就可观察到相差六个碱基的DNA Ladder现象(参见图4)。此外，Intergen公司还提供用酶联免疫法(ELISA)检测的试剂盒. 同样，这种检测方法也不专对细胞凋亡，检测结果也不纯反应细胞凋亡的发生
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