- ￥2890
- LSM Bio
- 0.31-20NG/ML
- 0.1NG/ML
- 进口
- ELI-52337h
- 2026年01月05日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.31-20NG/ML
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Src-like-adapter 2,SLA2
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.1NG/ML
- 规格：
96T
Human Src- like- adapter 2, SLA2 ELISA KIT
Product Name:Human Src- like- adapter 2, SLA2 ELISA KIT
Packing:96T
Catalog No.:ELI-52337h
Gene Name:SLA2
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Src-like-adapter 2(SLA2
Alternative Name:SLA2,Src-like-adapter 2(SLA2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Src- like- adapter 2, SLA2 ELISA KIT allows for the in vitro quantitative determination of Src-like-adapter 2(SLA2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Src- like- adapter 2, SLA2 ELISA KIT has been pre-coated with an Src-like-adapter 2(SLA2 antibody specific to Src-like-adapter 2(SLA2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Src-like-adapter 2(SLA2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Src-like-adapter 2(SLA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Src-like-adapter 2(SLA2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验上 Strep-tag 的 Fab 片段（Fab-Streps）特异性的结合到琼脂糖基质上。随后，加入全血或其他血液制剂流过柱子。目的细胞通过特异性的 Fab-Strep 作用从而结合到琼脂糖基质上，其他非目的细胞则有效的被洗去。最后，加入生物素使目的细胞从 Fab-Streps 上解离。至此，就已分离得到不带任何标记的、真实的目的细胞可用于下游的实验研究。 1. 实验流程 所需产品： CD3 Fab-TACS® Gravity Kit, human（IBA Lifesciences, Cat
Native Chromatin Preparation and Illumina/Solexa Library Construction
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有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂，增加了操作步骤，在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法：1， TORCH及传染病试剂盒（间接法），见2.2.32， TORCH-IgM捕获法特色：包被抗体，标记抗原原理：3， 细胞因子试剂盒采用的方法路线（ABC-ELISA）原理产品特色：采用ABC法，灵敏度更高，特异性更强。生物素抗体和酶联物是浓缩的，使用前需用相应的缓冲液稀释。酶联物可以通用
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