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Human UBE4A (Ubiquitin conjuga

tion factor E4 A) ELISA Kit (CUSTO
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  • ¥2890
  • LSM Bio
  • 0.15-10NG/ML
  • 0.078NG/ML
  • 进口
  • ELI-51351h
  • 2025年07月16日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 库存

      50

    • 供应商

      LSMBIO

    • 检测范围

      0.15-10NG/ML

    • 检测方法

      夹心法

    • 应用

      夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度

    • 适应物种

    • 标记物

      Human UBE4A (Ubiquitin conjugation factor E4 A) (CUSTO

    • 样本

      人血清、血浆或其它相关生物液体中天然和重组蛋白

    • 灵敏度

      0.078NG/ML

    • 规格

      96T

    Human UBE4A (Ubiquitin conjugation factor E4 A) ELISA Kit (CUSTOM)

    96 Tests

    Operating instructions

     

    FOR RESEARCH USE ONLY!

     

    Alternative name

    Human UBE4A ELISA KIT ,Human UBOX2 ELISA KIT ,Human UFD2 ELISA KIT ,Human ubiquitination factor E4A (UFD2 homolog yeast) ELISA KIT ,Human ubiquitination factor E4 ELISA KIT

     

    Intended use

    Human UBE4A ELISA kit allows for the in vitro quantitative determination of Human Ubiquitin conjugation factor E4 A concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

     

    Test principle

    Human UBE4A ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to UBE4A, During the reaction, UBE4A in the sample or standard competes with a fixed amount of biotin-labeled UBE4A for sites on a pre-coated Monoclonal antibody specific to UBE4A. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of UBE4A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

    Customized product    Min.Order 5kits

    Delivery time 4-6month

    Order Human UBE4A ELISA kit by email:

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    图标文献和实验
    该产品被引用文献
    Bioinformatics and experimental insights into F2RL1 as a key Publication: biomarker in cervical cancer diagnosis and prognosis. Dai, Y., Li, C., Cheng, S. et al. Sci Rep 15, 5228 (2025). doi.org/10.1038/s41598-025-89746-y
    相关实验
    • Studies of the Ubiquitin Proteasome System

      of Small Interfering RNAs (siRNAs) to Reduce Expression of Ubiquitin‐Proteasome System Proteins in Cells Basic Protocol 13: Localizing Degradation to the Proteasome The NEDD8 Conjugation System Basic Protocol 14: Purification of APP‐BP1/Uba3 Complex (E1

    • 《Nature》:用RNAi攻击胆固醇

      isolation kit, Qiagen), pooled mRNA for each treatment group. As well as GAPDH, factor VII, glucose-6-phosphatase and VEGF mRNAs were also used for normalization. ELISA was used to quantify the reduction of apoB-100 protein levels in mouse plasma

    • secondary antibody review -- data from 99 publications

      ref host class subclass conjugation experiment dilution detail supplier 1 goat     Alexa Fluor 488 immunocytochemistry 1:250 localization of tagged EVA-1 in 293T cell Molecular Probes 1 goat     Alexa Fluor 546 immunocytochemistry 1:250

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    文献支持
    Human UBE4A (Ubiquitin conjugation factor E4 A) ELISA Kit (CUSTO
    ¥2890