- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 国产
- ELI-28628h
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Ubiquitin conjugation factor E4 B,UBE4B
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Ubiquitin conjugation factor E4 B, UBE4B ELISA KIT
Product Name:Human Ubiquitin conjugation factor E4 B, UBE4B ELISA KIT
Packing:96T
Catalog No.:ELI-28628h
Gene Name:UBE4B
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Ubiquitin conjugation factor E4 B(UBE4B
Alternative Name:UBE4B,Ubiquitin conjugation factor E4 B(UBE4B
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Ubiquitin conjugation factor E4 B, UBE4B ELISA KIT allows for the in vitro quantitative determination of Ubiquitin conjugation factor E4 B(UBE4B concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Ubiquitin conjugation factor E4 B, UBE4B ELISA KIT has been pre-coated with an Ubiquitin conjugation factor E4 B(UBE4B antibody specific to Ubiquitin conjugation factor E4 B(UBE4B .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Ubiquitin conjugation factor E4 B(UBE4B and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Ubiquitin conjugation factor E4 B(UBE4B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Ubiquitin conjugation factor E4 B(UBE4B in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Studies of the Ubiquitin Proteasome System
of Small Interfering RNAs (siRNAs) to Reduce Expression of Ubiquitin‐Proteasome System Proteins in Cells Basic Protocol 13: Localizing Degradation to the Proteasome The NEDD8 Conjugation System Basic Protocol 14: Purification of APP‐BP1/Uba3 Complex (E1
this method will dilute the antibody by a factor of 2. Using a PD-10 column: a)Wash PD-10 column with 30mL of PBS. b) Add the 1mL FITC-Ig or Biotin Ig solution after the top of column is almost dry. Close stopcock to stop flow
Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
9. GlutaMAX™-I Supplement 10. Basic FGF, Recombinant Human 11. β-Mercaptoethanol, 1000X 12. Penicillin-Streptomycin, Liquid 13. Attachment Factor 14. TrypLE™ Select Cell Dissociation Reagent or 0.05% Trypsin/EDTA
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