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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10NG/ML
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Complement C1s subcomponent,C1S
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078NG/ML
- 规格:
96T
Human Complement C1s subcomponent, C1S ELISA KIT
Product Name:Human Complement C1s subcomponent, C1S ELISA KIT
Packing:96T
Catalog No.:ELI-50438h
Gene Name:C1S
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Complement C1s subcomponent(C1S
Alternative Name:C1S,Complement C1s subcomponent(C1S
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Complement C1s subcomponent, C1S ELISA KIT allows for the in vitro quantitative determination of Complement C1s subcomponent(C1S concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Complement C1s subcomponent, C1S ELISA KIT has been pre-coated with an Complement C1s subcomponent(C1S antibody specific to Complement C1s subcomponent(C1S .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Complement C1s subcomponent(C1S and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Complement C1s subcomponent(C1S, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Complement C1s subcomponent(C1S in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验C1r and C1s are the proteases responsible for the activation and proteolytic activity of the C1 complex of the classical complement pathway, respectively. They are assembled into a Ca2+ -dependent C1s–C1r–C1r–C1s tetramer which in turn
亚单位(链)及分子量(kDa):1条 激活产物:C1r� 生物学活性:丝氨酸蛋白酶,裂解C1s 补体成分:C1s 血清浓度(μg/ml):50 分子量(kDa):85 亚单位(链)及分子量(kDa):1条 激活产物:C1s� 生物学活性:丝氨酸蛋白酶,裂解C4、C2 ELISA KIT
Evaluation of Complement Function by ELISA
Evaluation of total complement function in human serum is an essential component of laboratory diagnostics of the human complement system. During recent years, established hemolytic assays for classical pathway and alternative pathway
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