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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSMBIO
- 检测范围:
0.31-20NG/ML
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Histone-arginine methyltransferase CARM1,CARM1
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.1NG/ML
- 规格:
96T
Human Histone- arginine methyltransferase CARM1, CARM1 ELISA KIT
Product Name:Human Histone- arginine methyltransferase CARM1, CARM1 ELISA KIT
Packing:96T
Catalog No.:ELI-50387h
Gene Name:CARM1
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Histone-arginine methyltransferase CARM1(CARM1
Alternative Name:CARM1,Histone-arginine methyltransferase CARM1(CARM1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Histone- arginine methyltransferase CARM1, CARM1 ELISA KIT allows for the in vitro quantitative determination of Histone-arginine methyltransferase CARM1(CARM1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Histone- arginine methyltransferase CARM1, CARM1 ELISA KIT has been pre-coated with an Histone-arginine methyltransferase CARM1(CARM1 antibody specific to Histone-arginine methyltransferase CARM1(CARM1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Histone-arginine methyltransferase CARM1(CARM1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Histone-arginine methyltransferase CARM1(CARM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Histone-arginine methyltransferase CARM1(CARM1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验by 35S-methionine For more details about procedure and controls see: Kzhyshkowska J et al, Heterogeneous nuclear ribonucleoprotein E1B-AP5 is methylated in its Arg-Gly-Gly (RGG) box and interacts with human arginine methyltransferase HRMT1L1.
Measurement of Arginine Metabolites: Regulators of Nitric Oxide Metabolism
Abstract Table of Contents Materials Figures Literature Cited Abstract Arginine
, A.D., Rossi, J.J. and Morris, K.V. (2005) The antisense strand of small interfering RNAs directs histone methylation and transcriptional gene silencing in human cells, RNA, 12(2): 256-262.
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