Target Protein Name:Bcl10-interacting CARD protein(C9orf89
Alternative Name:C9orf89,Bcl10-interacting CARD protein(C9orf89 Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit Product Description:Human Bcl10- interacting CARD protein, C9orf89 ELISA KIT allows for the in vitro quantitative determination of Bcl10-interacting CARD protein(C9orf89 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle: The microtiter plate provided in Human Bcl10- interacting CARD protein, C9orf89 ELISA KIT has been pre-coated with an Bcl10-interacting CARD protein(C9orf89 antibody specific to Bcl10-interacting CARD protein(C9orf89 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bcl10-interacting CARD protein(C9orf89 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bcl10-interacting CARD protein(C9orf89, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bcl10-interacting CARD protein(C9orf89 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!