- ￥2890
- LSM Bio
- 0.31-20ng/Ml
- 0.1ng/Ml
- 进口
- ELI-48182h
- 2025年12月21日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSMBIO
- 检测范围：
0.31-20ng/Ml
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Hedgehog-interacting protein,HHIP
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.1ng/Ml
- 规格：
96T
Human Hedgehog- interacting protein, HHIP ELISA KIT
Product Name:Human Hedgehog- interacting protein, HHIP ELISA KIT
Packing:96T
Catalog No.:ELI-48182h
Gene Name:HHIP
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Hedgehog-interacting protein(HHIP
Alternative Name:HHIP,Hedgehog-interacting protein(HHIP
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Hedgehog- interacting protein, HHIP ELISA KIT allows for the in vitro quantitative determination of Hedgehog-interacting protein(HHIP concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Hedgehog- interacting protein, HHIP ELISA KIT has been pre-coated with an Hedgehog-interacting protein(HHIP antibody specific to Hedgehog-interacting protein(HHIP .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Hedgehog-interacting protein(HHIP and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Hedgehog-interacting protein(HHIP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Hedgehog-interacting protein(HHIP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Purifying the Hedgehog Protein and its Variants
to human Indian and Desert hedgehog N-terminal signaling proteins, and therefore should be useful for hedgehog proteins from other species.
Interaction Trap/Two‐Hybrid System to Identify Interacting Proteins
pentagon, bait protein; open rectangle, noninteracting library protein; shaded box, activator protein (acid blob in Fig. ); open chevron, interacting library protein. View Image
technical steps to recover uPA and PAI-1 protein from archived breast cancer tissues and to quantitatively determine uPA and PAI-1 protein content in tumor tissue extracts by enzyme-linked immunosorbent assay (ELISA) are described in detail. The technical
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