- ￥2890
- LSM Bio
- 0.15-10NG/ML
- 0.078NG/ML
- 进口
- ELI-49385h
- 2025年07月07日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10NG/ML
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Box C/D snoRNA protein 1,ZNHIT6
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078NG/ML
- 规格：
96T
Human Box C/D snoRNA protein 1, ZNHIT6 ELISA KIT
Product Name:Human Box C/D snoRNA protein 1, ZNHIT6 ELISA KIT
Packing:96T
Catalog No.:ELI-49385h
Gene Name:ZNHIT6
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Box C(D snoRNA protein 1(ZNHIT6
Alternative Name:ZNHIT6,Box C(D snoRNA protein 1(ZNHIT6
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Box C/D snoRNA protein 1, ZNHIT6 ELISA KIT allows for the in vitro quantitative determination of Box C(D snoRNA protein 1(ZNHIT6 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Box C/D snoRNA protein 1, ZNHIT6 ELISA KIT has been pre-coated with an Box C(D snoRNA protein 1(ZNHIT6 antibody specific to Box C(D snoRNA protein 1(ZNHIT6 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Box C(D snoRNA protein 1(ZNHIT6 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Box C(D snoRNA protein 1(ZNHIT6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Box C(D snoRNA protein 1(ZNHIT6 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验体蛋白，但下游外显子则缺少明显的开放读码框，取而代之的是下游的内含子内编码了至少6 种b o x C /D snoRNA，其中，24 个HBII-85 和47 个HBII-52 分子，分别位于其中的两段串联重复序列。而这段印迹区可能与Prader-Willi(PWS)或Angelman综合征有关[11-13]。 大鼠中的RBII-36 同样具有串联重复序列，它是由非蛋白质编码的基因Bsr 所编码的。在人和小鼠相应的同源区域，也包含有与印迹相关的组织特异性box C/D snoRNA
is an indirect expression of the monoclonal antibody amount in the sample.Methods1 Obtain a 96-well plate and rinse 3x with PBS.2 Add 50 µl of a 1 mg/ml of antigen (venom, serum, or fraction), incubate for 30 min at 37°C.3 Rinse with PBS 3x.4 Add 5% powdered milk
Typical modification sites: RGG box or RXR sequence motifs R-arginine, G-glycine, X-any aminoacid. Enzymes catalysing protein arginine methylation: PRMT1 from rat and the human homologue HRMT1L2, human PRMT2 (HRMT1L1), rat and human PRMT
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