- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 国产
- ELI-12992h
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Helix-loop-helix protein 2,NHLH2
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Helix- loop- helix protein 2, NHLH2 ELISA KIT
Product Name:Human Helix- loop- helix protein 2, NHLH2 ELISA KIT
Packing:96T
Catalog No.:ELI-12992h
Gene Name:NHLH2
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Helix-loop-helix protein 2(NHLH2
Alternative Name:NHLH2,Helix-loop-helix protein 2(NHLH2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Helix- loop- helix protein 2, NHLH2 ELISA KIT allows for the in vitro quantitative determination of Helix-loop-helix protein 2(NHLH2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Helix- loop- helix protein 2, NHLH2 ELISA KIT has been pre-coated with an Helix-loop-helix protein 2(NHLH2 antibody specific to Helix-loop-helix protein 2(NHLH2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Helix-loop-helix protein 2(NHLH2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Helix-loop-helix protein 2(NHLH2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Helix-loop-helix protein 2(NHLH2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Identification of Protein Interactions by Far Western Analysis
protein of interest cloned into an in vitro expression vector In vitro transcription/translation kit (Promega) 10 mCi/ml 35
was found near the termination codon of Hes1 (77% homology)--a basic helix-loop-helix transcriptional repressor that is part of the Notch signaling pathway.To determine whether the Hes1 mRNA is actually a target for miRNA-23, the authors used NT2 cells as a model system. NT
酶解再向β折叠链的构象转变成无活力的裂解型(cleaved-form, C型)［11］. L型抑制剂可以通过变性和复性方法转变成A型, 然后又慢慢变成L型. 流感病毒血细胞凝集素(influenza hemagglutinin, HA)在pH诱导下的结构变化与细胞膜融合功能紧密相关的. HA在pH 7.0时, 其空间结构是由helix-loop-helix的头状单体结构域组成的三聚体蛋白(HA-N); 可在低pH(pH＜5)条件下, 中间Loop区域转化为螺旋结构并与另两段螺旋连接共同形成一股长螺旋
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