- ￥2890
- LSM Bio
- 0.31-20ng/Ml
- 0.1ng/Ml
- 进口
- ELI-45564h
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSMBIO
- 检测范围：
0.31-20ng/Ml
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Next to BRCA1 gene 1 protein,NBR1
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.1ng/Ml
- 规格：
96T
Human Next to BRCA1 gene 1 protein, NBR1 ELISA KIT
Product Name:Human Next to BRCA1 gene 1 protein, NBR1 ELISA KIT
Packing:96T
Catalog No.:ELI-45564h
Gene Name:NBR1
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Next to BRCA1 gene 1 protein(NBR1
Alternative Name:NBR1,Next to BRCA1 gene 1 protein(NBR1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Next to BRCA1 gene 1 protein, NBR1 ELISA KIT allows for the in vitro quantitative determination of Next to BRCA1 gene 1 protein(NBR1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Next to BRCA1 gene 1 protein, NBR1 ELISA KIT has been pre-coated with an Next to BRCA1 gene 1 protein(NBR1 antibody specific to Next to BRCA1 gene 1 protein(NBR1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Next to BRCA1 gene 1 protein(NBR1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Next to BRCA1 gene 1 protein(NBR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Next to BRCA1 gene 1 protein(NBR1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验的原理用于分子诊断方面：TrimGen公司的KRAS Mutation Detection Kit在96孔板里一次能实现7/8个KRAS突变位点的检测。 另外基于相同原理的技术还有PPT-ELISA。这是用来筛截短突变的，相关文章见：1，Rapid screen for truncating ATM mutations by PTT-ELISA . 2，An ELISA-based high throughput protein truncation test
should be analyzed. Protein levels can be monitored by Western blot, immunofluorescence, ELISA or other means. (Protein can be isolated using Ambion's PARIS™ Kit.) For quantitating mRNA levels, however, qRT-PCR is again the preferred choice.An example
protein levels should be analyzed. Protein levels can be monitored by Western blot, immunofluorescence, ELISA or other means. (Protein can be isolated using Ambion's PARIS™ Kit.) For quantitating mRNA levels, however, qRT-PCR is again the preferred choice
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