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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ml
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Pituitary adenylate cyclase-activating polypeptide type I
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Pituitary adenylate cyclase- activating polypeptide type I receptor, ADCYAP1R1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
ADCYAP1R1,PACAP-R1; PAC1; PAC1R; PACAPR; PACAPRI; PACAP-R-1,PACAP receptor 1; PACAP type I receptor; pituitary adenylate cyclase activating polypeptide 1 receptor type I Hiphop; pituitary adenylate cyclase-activating polypeptide type I receptor; adenylate cyclase activating polypeptide 1 (pituitary) receptor type I
Search name
Human ADCYAP1R1 ELISA KIT ,Human PACAP-R1 ELISA KIT ,Human PAC1 ELISA KIT ,Human PAC1R ELISA KIT ,Human PACAPR ELISA KIT ,Human PACAPRI ELISA KIT ,Human PACAP-R-1 ELISA KIT ,Human PACAP receptor 1 ELISA KIT ,Human PACAP type I receptor ELISA KIT ,Human pituitary adenylate cyclase activating polypeptide 1 receptor type I Hiphop ELISA KIT ,Human pituitary adenylate cyclase-activating polypeptide type I receptor ELISA KIT ,Human adenylate cyclase activating polypeptide 1 (pituitary) receptor type I ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of human Pituitary adenylate cyclase-activating polypeptide type i receptor, PACAP type I receptor concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to PACAP type I receptor. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PACAP type I receptor and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PACAP type I receptor, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PACAP type I receptor in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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